No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers

Novel viruses might be responsible for numerous disease cases with unknown etiology. In this study, we screened 1800 nasopharyngeal samples from adult outpatients with respiratory disease symptoms and healthy individuals. We employed a reverse transcription (RT)-PCR assay and CODEHOP-based primers (...

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Autores principales: Zlateva, Kalina T., Coenjaerts, Frank E. J., Crusio, Kelly M., Lammens, Christine, Leus, Frank, Viveen, Marco, Ieven, Margareta, Spaan, Willy J. M., Claas, Eric C. J., Gorbalenya, Alexander E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2012
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087030/
https://www.ncbi.nlm.nih.gov/pubmed/23053517
http://dx.doi.org/10.1007/s00705-012-1487-4
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author Zlateva, Kalina T.
Coenjaerts, Frank E. J.
Crusio, Kelly M.
Lammens, Christine
Leus, Frank
Viveen, Marco
Ieven, Margareta
Spaan, Willy J. M.
Claas, Eric C. J.
Gorbalenya, Alexander E.
author_facet Zlateva, Kalina T.
Coenjaerts, Frank E. J.
Crusio, Kelly M.
Lammens, Christine
Leus, Frank
Viveen, Marco
Ieven, Margareta
Spaan, Willy J. M.
Claas, Eric C. J.
Gorbalenya, Alexander E.
author_sort Zlateva, Kalina T.
collection PubMed
description Novel viruses might be responsible for numerous disease cases with unknown etiology. In this study, we screened 1800 nasopharyngeal samples from adult outpatients with respiratory disease symptoms and healthy individuals. We employed a reverse transcription (RT)-PCR assay and CODEHOP-based primers (CT12-mCODEHOP) previously developed to recognize known and unknown corona- and toroviruses. The CT12-mCODEHOP assay detected 42.0 % (29/69) of samples positive for human coronaviruses (HCoV), including HCoV-229 (1/16), HCoV-NL63 (9/17), and HCoV-OC43 (19/36), and additionally HCoV-HKU1 (3), which was not targeted by the diagnostic real-time PCR assays. No other coronaviruses were identified in the analyzed samples.
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spelling pubmed-70870302020-03-23 No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers Zlateva, Kalina T. Coenjaerts, Frank E. J. Crusio, Kelly M. Lammens, Christine Leus, Frank Viveen, Marco Ieven, Margareta Spaan, Willy J. M. Claas, Eric C. J. Gorbalenya, Alexander E. Arch Virol Brief Report Novel viruses might be responsible for numerous disease cases with unknown etiology. In this study, we screened 1800 nasopharyngeal samples from adult outpatients with respiratory disease symptoms and healthy individuals. We employed a reverse transcription (RT)-PCR assay and CODEHOP-based primers (CT12-mCODEHOP) previously developed to recognize known and unknown corona- and toroviruses. The CT12-mCODEHOP assay detected 42.0 % (29/69) of samples positive for human coronaviruses (HCoV), including HCoV-229 (1/16), HCoV-NL63 (9/17), and HCoV-OC43 (19/36), and additionally HCoV-HKU1 (3), which was not targeted by the diagnostic real-time PCR assays. No other coronaviruses were identified in the analyzed samples. Springer Vienna 2012-09-28 2013 /pmc/articles/PMC7087030/ /pubmed/23053517 http://dx.doi.org/10.1007/s00705-012-1487-4 Text en © Springer-Verlag Wien 2012 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Zlateva, Kalina T.
Coenjaerts, Frank E. J.
Crusio, Kelly M.
Lammens, Christine
Leus, Frank
Viveen, Marco
Ieven, Margareta
Spaan, Willy J. M.
Claas, Eric C. J.
Gorbalenya, Alexander E.
No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers
title No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers
title_full No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers
title_fullStr No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers
title_full_unstemmed No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers
title_short No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers
title_sort no novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide codehop-based primers
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087030/
https://www.ncbi.nlm.nih.gov/pubmed/23053517
http://dx.doi.org/10.1007/s00705-012-1487-4
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