Detection of canine astrovirus in dogs with diarrhea in Japan

Canine astrovirus (CAstV) is the causative agent of gastroenteritis in dogs. We collected rectal swabs from dogs with or without diarrhea symptoms in Japan and examined the feces for the presence of CAstV by RT-PCR with primers based on a conserved region of the ORF1b gene. The ORF1b gene of CAstV w...

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Autores principales: Takano, Tomomi, Takashina, Midori, Doki, Tomoyoshi, Hohdatsu, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2015
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087093/
https://www.ncbi.nlm.nih.gov/pubmed/25824600
http://dx.doi.org/10.1007/s00705-015-2405-3
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author Takano, Tomomi
Takashina, Midori
Doki, Tomoyoshi
Hohdatsu, Tsutomu
author_facet Takano, Tomomi
Takashina, Midori
Doki, Tomoyoshi
Hohdatsu, Tsutomu
author_sort Takano, Tomomi
collection PubMed
description Canine astrovirus (CAstV) is the causative agent of gastroenteritis in dogs. We collected rectal swabs from dogs with or without diarrhea symptoms in Japan and examined the feces for the presence of CAstV by RT-PCR with primers based on a conserved region of the ORF1b gene. The ORF1b gene of CAstV was not detected in the 42 dogs without clinical illness but was present in three pups out of the 31 dogs with diarrhea symptoms. Based on the full-length capsid protein, the CAstV KU-D4-12 strain that we detected in this study shared high homology with the novel virulent CAstV VM-2011 strain.
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spelling pubmed-70870932020-03-23 Detection of canine astrovirus in dogs with diarrhea in Japan Takano, Tomomi Takashina, Midori Doki, Tomoyoshi Hohdatsu, Tsutomu Arch Virol Brief Report Canine astrovirus (CAstV) is the causative agent of gastroenteritis in dogs. We collected rectal swabs from dogs with or without diarrhea symptoms in Japan and examined the feces for the presence of CAstV by RT-PCR with primers based on a conserved region of the ORF1b gene. The ORF1b gene of CAstV was not detected in the 42 dogs without clinical illness but was present in three pups out of the 31 dogs with diarrhea symptoms. Based on the full-length capsid protein, the CAstV KU-D4-12 strain that we detected in this study shared high homology with the novel virulent CAstV VM-2011 strain. Springer Vienna 2015-04-01 2015 /pmc/articles/PMC7087093/ /pubmed/25824600 http://dx.doi.org/10.1007/s00705-015-2405-3 Text en © Springer-Verlag Wien 2015 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Takano, Tomomi
Takashina, Midori
Doki, Tomoyoshi
Hohdatsu, Tsutomu
Detection of canine astrovirus in dogs with diarrhea in Japan
title Detection of canine astrovirus in dogs with diarrhea in Japan
title_full Detection of canine astrovirus in dogs with diarrhea in Japan
title_fullStr Detection of canine astrovirus in dogs with diarrhea in Japan
title_full_unstemmed Detection of canine astrovirus in dogs with diarrhea in Japan
title_short Detection of canine astrovirus in dogs with diarrhea in Japan
title_sort detection of canine astrovirus in dogs with diarrhea in japan
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087093/
https://www.ncbi.nlm.nih.gov/pubmed/25824600
http://dx.doi.org/10.1007/s00705-015-2405-3
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