Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs

In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a h...

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Detalles Bibliográficos
Autores principales: Ntafis, Vasileios, Xylouri, Eftychia, Mari, Viviana, Papanastassopoulou, Maria, Papaioannou, Nikolaos, Thomas, Angelos, Buonavoglia, Canio, Decaro, Nicola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2011
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087105/
https://www.ncbi.nlm.nih.gov/pubmed/22002680
http://dx.doi.org/10.1007/s00705-011-1141-6
Descripción
Sumario:In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region.

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