Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs

In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a h...

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Autores principales: Ntafis, Vasileios, Xylouri, Eftychia, Mari, Viviana, Papanastassopoulou, Maria, Papaioannou, Nikolaos, Thomas, Angelos, Buonavoglia, Canio, Decaro, Nicola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2011
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087105/
https://www.ncbi.nlm.nih.gov/pubmed/22002680
http://dx.doi.org/10.1007/s00705-011-1141-6
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author Ntafis, Vasileios
Xylouri, Eftychia
Mari, Viviana
Papanastassopoulou, Maria
Papaioannou, Nikolaos
Thomas, Angelos
Buonavoglia, Canio
Decaro, Nicola
author_facet Ntafis, Vasileios
Xylouri, Eftychia
Mari, Viviana
Papanastassopoulou, Maria
Papaioannou, Nikolaos
Thomas, Angelos
Buonavoglia, Canio
Decaro, Nicola
author_sort Ntafis, Vasileios
collection PubMed
description In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region.
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spelling pubmed-70871052020-03-23 Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs Ntafis, Vasileios Xylouri, Eftychia Mari, Viviana Papanastassopoulou, Maria Papaioannou, Nikolaos Thomas, Angelos Buonavoglia, Canio Decaro, Nicola Arch Virol Brief Report In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region. Springer Vienna 2011-10-16 2012 /pmc/articles/PMC7087105/ /pubmed/22002680 http://dx.doi.org/10.1007/s00705-011-1141-6 Text en © Springer-Verlag 2011 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Ntafis, Vasileios
Xylouri, Eftychia
Mari, Viviana
Papanastassopoulou, Maria
Papaioannou, Nikolaos
Thomas, Angelos
Buonavoglia, Canio
Decaro, Nicola
Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs
title Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs
title_full Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs
title_fullStr Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs
title_full_unstemmed Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs
title_short Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs
title_sort molecular characterization of a canine coronavirus na/09 strain detected in a dog’s organs
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087105/
https://www.ncbi.nlm.nih.gov/pubmed/22002680
http://dx.doi.org/10.1007/s00705-011-1141-6
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