Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China

Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006–2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucle...

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Autores principales: Chen, Jianfei, Liu, Xiaozhen, Lang, Hongwu, Wang, Zhongtian, Shi, Da, Shi, Hongyan, Zhang, Xin, Feng, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2013
Materias:
Annotated Sequence Record
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087116/
https://www.ncbi.nlm.nih.gov/pubmed/23381394
http://dx.doi.org/10.1007/s00705-013-1608-8
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author Chen, Jianfei
Liu, Xiaozhen
Lang, Hongwu
Wang, Zhongtian
Shi, Da
Shi, Hongyan
Zhang, Xin
Feng, Li
author_facet Chen, Jianfei
Liu, Xiaozhen
Lang, Hongwu
Wang, Zhongtian
Shi, Da
Shi, Hongyan
Zhang, Xin
Feng, Li
author_sort Chen, Jianfei
collection PubMed
description Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006–2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups.
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spelling pubmed-70871162020-03-23 Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China Chen, Jianfei Liu, Xiaozhen Lang, Hongwu Wang, Zhongtian Shi, Da Shi, Hongyan Zhang, Xin Feng, Li Arch Virol Annotated Sequence Record Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006–2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups. Springer Vienna 2013-02-05 2013 /pmc/articles/PMC7087116/ /pubmed/23381394 http://dx.doi.org/10.1007/s00705-013-1608-8 Text en © Springer-Verlag Wien 2013 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Annotated Sequence Record
Chen, Jianfei
Liu, Xiaozhen
Lang, Hongwu
Wang, Zhongtian
Shi, Da
Shi, Hongyan
Zhang, Xin
Feng, Li
Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China
title Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China
title_full Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China
title_fullStr Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China
title_full_unstemmed Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China
title_short Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China
title_sort genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in china
topic Annotated Sequence Record
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087116/
https://www.ncbi.nlm.nih.gov/pubmed/23381394
http://dx.doi.org/10.1007/s00705-013-1608-8
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