A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection

The one-step polymerase chain reaction (one-step PCR) detection assay is an innovative PCR detection method, eliminating nucleic acid extraction steps, in which samples can be directly added to PCR reagents for testing. For simultaneous detection of CDV and CCoV, a sensitive and specific one-step du...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jing, Luo, Yakun, Liang, Lin, Li, Jinxiang, Cui, Shangjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2018
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087121/
https://www.ncbi.nlm.nih.gov/pubmed/30141131
http://dx.doi.org/10.1007/s00705-018-3982-8
_version_ 1783509271258857472
author Wang, Jing
Luo, Yakun
Liang, Lin
Li, Jinxiang
Cui, Shangjin
author_facet Wang, Jing
Luo, Yakun
Liang, Lin
Li, Jinxiang
Cui, Shangjin
author_sort Wang, Jing
collection PubMed
description The one-step polymerase chain reaction (one-step PCR) detection assay is an innovative PCR detection method, eliminating nucleic acid extraction steps, in which samples can be directly added to PCR reagents for testing. For simultaneous detection of CDV and CCoV, a sensitive and specific one-step duplex PCR (one-step dPCR) assay was developed with two pairs of primers that were designed based on H and M gene sequences of CDV and CCoV, respectively. The one-step dPCR with optimized detection conditions has high specificity and sensitivity; independent sequencing assays further verified these results.
format Online
Article
Text
id pubmed-7087121
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-70871212020-03-23 A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection Wang, Jing Luo, Yakun Liang, Lin Li, Jinxiang Cui, Shangjin Arch Virol Brief Report The one-step polymerase chain reaction (one-step PCR) detection assay is an innovative PCR detection method, eliminating nucleic acid extraction steps, in which samples can be directly added to PCR reagents for testing. For simultaneous detection of CDV and CCoV, a sensitive and specific one-step duplex PCR (one-step dPCR) assay was developed with two pairs of primers that were designed based on H and M gene sequences of CDV and CCoV, respectively. The one-step dPCR with optimized detection conditions has high specificity and sensitivity; independent sequencing assays further verified these results. Springer Vienna 2018-08-23 2018 /pmc/articles/PMC7087121/ /pubmed/30141131 http://dx.doi.org/10.1007/s00705-018-3982-8 Text en © Springer-Verlag GmbH Austria, part of Springer Nature 2018 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Wang, Jing
Luo, Yakun
Liang, Lin
Li, Jinxiang
Cui, Shangjin
A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection
title A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection
title_full A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection
title_fullStr A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection
title_full_unstemmed A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection
title_short A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection
title_sort fast and simple one-step duplex pcr assay for canine distemper virus (cdv) and canine coronavirus (ccov) detection
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087121/
https://www.ncbi.nlm.nih.gov/pubmed/30141131
http://dx.doi.org/10.1007/s00705-018-3982-8
work_keys_str_mv AT wangjing afastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection
AT luoyakun afastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection
AT lianglin afastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection
AT lijinxiang afastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection
AT cuishangjin afastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection
AT wangjing fastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection
AT luoyakun fastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection
AT lianglin fastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection
AT lijinxiang fastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection
AT cuishangjin fastandsimpleonestepduplexpcrassayforcaninedistemperviruscdvandcaninecoronavirusccovdetection

Ejemplares similares