Cargando…

Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection

 Post-weaning multisystemic wasting syndrome (PMWS) is a recently identified disease of pigs linked to the emergence of a new porcine circovirus (PCV2). We report here the characterization of immunorelevant linear B-cell epitopes of the Open Reading Frame 2-encoded protein (Orf2) from PCV2 by an enz...

Descripción completa

Detalles Bibliográficos
Autores principales: Truong, C., Mahe, D., Blanchard, P., Le Dimna, M., Madec, F., Jestin, A., Albina, E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087129/
https://www.ncbi.nlm.nih.gov/pubmed/11504425
http://dx.doi.org/10.1007/s007050170115
_version_ 1783509273089671168
author Truong, C.
Mahe, D.
Blanchard, P.
Le Dimna, M.
Madec, F.
Jestin, A.
Albina, E.
author_facet Truong, C.
Mahe, D.
Blanchard, P.
Le Dimna, M.
Madec, F.
Jestin, A.
Albina, E.
author_sort Truong, C.
collection PubMed
description  Post-weaning multisystemic wasting syndrome (PMWS) is a recently identified disease of pigs linked to the emergence of a new porcine circovirus (PCV2). We report here the characterization of immunorelevant linear B-cell epitopes of the Open Reading Frame 2-encoded protein (Orf2) from PCV2 by an enzyme-linked immunosorbent assay (ELISA) using experimental antisera collected from pigs inoculated with a PCV2 isolate. Two epitopes spanning residues 69 to 83 and 117 to 131 were specific to PCV2. Antibodies to the 117 to 131 epitope (B-133) were detected in 22% and 100% of specific pathogen-free (SPF) pig sera 6 and 11 weeks post inoculation, respectively. Cross-sectional studies performed with field sera collected from PMWS-affected herds showed B-133 antibodies in 5% of 8 to 10 week-old pigs, 38% of 13–14 week-old pigs, 62% of 16 to 19 week-old pigs, 56% of 20 to 25 week-old pigs and 45% of 26 to 31 week-old pigs. All these data suggest that epitope B-133 is a serological marker of PCV2 infection that could be used for the detection of PCV2 antibody response.
format Online
Article
Text
id pubmed-7087129
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-70871292020-03-23 Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection Truong, C. Mahe, D. Blanchard, P. Le Dimna, M. Madec, F. Jestin, A. Albina, E. Arch Virol Article  Post-weaning multisystemic wasting syndrome (PMWS) is a recently identified disease of pigs linked to the emergence of a new porcine circovirus (PCV2). We report here the characterization of immunorelevant linear B-cell epitopes of the Open Reading Frame 2-encoded protein (Orf2) from PCV2 by an enzyme-linked immunosorbent assay (ELISA) using experimental antisera collected from pigs inoculated with a PCV2 isolate. Two epitopes spanning residues 69 to 83 and 117 to 131 were specific to PCV2. Antibodies to the 117 to 131 epitope (B-133) were detected in 22% and 100% of specific pathogen-free (SPF) pig sera 6 and 11 weeks post inoculation, respectively. Cross-sectional studies performed with field sera collected from PMWS-affected herds showed B-133 antibodies in 5% of 8 to 10 week-old pigs, 38% of 13–14 week-old pigs, 62% of 16 to 19 week-old pigs, 56% of 20 to 25 week-old pigs and 45% of 26 to 31 week-old pigs. All these data suggest that epitope B-133 is a serological marker of PCV2 infection that could be used for the detection of PCV2 antibody response. Springer-Verlag 2001 /pmc/articles/PMC7087129/ /pubmed/11504425 http://dx.doi.org/10.1007/s007050170115 Text en © Springer-Verlag/Wien 2001 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Truong, C.
Mahe, D.
Blanchard, P.
Le Dimna, M.
Madec, F.
Jestin, A.
Albina, E.
Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection
title Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection
title_full Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection
title_fullStr Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection
title_full_unstemmed Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection
title_short Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection
title_sort identification of an immunorelevant orf2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087129/
https://www.ncbi.nlm.nih.gov/pubmed/11504425
http://dx.doi.org/10.1007/s007050170115
work_keys_str_mv AT truongc identificationofanimmunorelevantorf2epitopefromporcinecircovirustype2asaserologicalmarkerforexperimentalandnaturalinfection
AT mahed identificationofanimmunorelevantorf2epitopefromporcinecircovirustype2asaserologicalmarkerforexperimentalandnaturalinfection
AT blanchardp identificationofanimmunorelevantorf2epitopefromporcinecircovirustype2asaserologicalmarkerforexperimentalandnaturalinfection
AT ledimnam identificationofanimmunorelevantorf2epitopefromporcinecircovirustype2asaserologicalmarkerforexperimentalandnaturalinfection
AT madecf identificationofanimmunorelevantorf2epitopefromporcinecircovirustype2asaserologicalmarkerforexperimentalandnaturalinfection
AT jestina identificationofanimmunorelevantorf2epitopefromporcinecircovirustype2asaserologicalmarkerforexperimentalandnaturalinfection
AT albinae identificationofanimmunorelevantorf2epitopefromporcinecircovirustype2asaserologicalmarkerforexperimentalandnaturalinfection