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High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus

Rabbits are widely used as models in biological research, and the pathogen status of rabbits used in studies can directly affect the results of experiments. Serological surveillance is the common monitoring method used in laboratory animals. A rapid, sensitive, and cost-effective high-throughput Lum...

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Autores principales: Wu, Miaoli, Ma, Lei, Cong, Feng, Zhu, Yujun, Xu, Fengjiao, Lian, Yuexiao, Huang, Bihong, Xiao, Li, Chen, Meili, Zhang, Yu, Huang, Ren, Guo, Pengju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087182/
https://www.ncbi.nlm.nih.gov/pubmed/30982935
http://dx.doi.org/10.1007/s00705-019-04226-9
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author Wu, Miaoli
Ma, Lei
Cong, Feng
Zhu, Yujun
Xu, Fengjiao
Lian, Yuexiao
Huang, Bihong
Xiao, Li
Chen, Meili
Zhang, Yu
Huang, Ren
Guo, Pengju
author_facet Wu, Miaoli
Ma, Lei
Cong, Feng
Zhu, Yujun
Xu, Fengjiao
Lian, Yuexiao
Huang, Bihong
Xiao, Li
Chen, Meili
Zhang, Yu
Huang, Ren
Guo, Pengju
author_sort Wu, Miaoli
collection PubMed
description Rabbits are widely used as models in biological research, and the pathogen status of rabbits used in studies can directly affect the results of experiments. Serological surveillance is the common monitoring method used in laboratory animals. A rapid, sensitive, and cost-effective high-throughput Luminex xMAP assay could be an attractive alternative to labor-intensive enzyme-linked immunosorbent assay (ELISA) methods. In this study, recombinant proteins from rabbit hemorrhagic disease virus and rabbit rotavirus and whole viral lysates of Sendai virus were used as coating antigens in an xMAP assay for the simultaneous detection of antibodies against these pathogens. The xMAP assay showed high specificity, with no cross-reaction with other pathogens. The coefficient of variation for intra-assay and inter-assay comparisons was less than 3% and 4%, respectively, indicating good repeatability and stability of the assay. The xMAP assay exhibited similar limits of detection for rabbit hemorrhagic virus and Sendai virus and was less sensitive for the detection of rabbit rotavirus when compared with commercial ELISA kits. A total of 52 clinical samples were tested simultaneously using both the xMAP assay and ELISA kits. The results obtained using these two methods were 100% coincident. In summary, the novel xMAP assay offers an alternative choice for rapid and sensitive high-throughput detection of antibodies in rabbit serum and can be used as a daily monitoring tool for laboratory animals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-019-04226-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-70871822020-03-23 High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus Wu, Miaoli Ma, Lei Cong, Feng Zhu, Yujun Xu, Fengjiao Lian, Yuexiao Huang, Bihong Xiao, Li Chen, Meili Zhang, Yu Huang, Ren Guo, Pengju Arch Virol Original Article Rabbits are widely used as models in biological research, and the pathogen status of rabbits used in studies can directly affect the results of experiments. Serological surveillance is the common monitoring method used in laboratory animals. A rapid, sensitive, and cost-effective high-throughput Luminex xMAP assay could be an attractive alternative to labor-intensive enzyme-linked immunosorbent assay (ELISA) methods. In this study, recombinant proteins from rabbit hemorrhagic disease virus and rabbit rotavirus and whole viral lysates of Sendai virus were used as coating antigens in an xMAP assay for the simultaneous detection of antibodies against these pathogens. The xMAP assay showed high specificity, with no cross-reaction with other pathogens. The coefficient of variation for intra-assay and inter-assay comparisons was less than 3% and 4%, respectively, indicating good repeatability and stability of the assay. The xMAP assay exhibited similar limits of detection for rabbit hemorrhagic virus and Sendai virus and was less sensitive for the detection of rabbit rotavirus when compared with commercial ELISA kits. A total of 52 clinical samples were tested simultaneously using both the xMAP assay and ELISA kits. The results obtained using these two methods were 100% coincident. In summary, the novel xMAP assay offers an alternative choice for rapid and sensitive high-throughput detection of antibodies in rabbit serum and can be used as a daily monitoring tool for laboratory animals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-019-04226-9) contains supplementary material, which is available to authorized users. Springer Vienna 2019-04-15 2019 /pmc/articles/PMC7087182/ /pubmed/30982935 http://dx.doi.org/10.1007/s00705-019-04226-9 Text en © Springer-Verlag GmbH Austria, part of Springer Nature 2019 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Wu, Miaoli
Ma, Lei
Cong, Feng
Zhu, Yujun
Xu, Fengjiao
Lian, Yuexiao
Huang, Bihong
Xiao, Li
Chen, Meili
Zhang, Yu
Huang, Ren
Guo, Pengju
High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus
title High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus
title_full High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus
title_fullStr High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus
title_full_unstemmed High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus
title_short High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus
title_sort high-throughput luminex xmap assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, sendai virus and rabbit rotavirus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087182/
https://www.ncbi.nlm.nih.gov/pubmed/30982935
http://dx.doi.org/10.1007/s00705-019-04226-9
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