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Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus
The coronavirus spike protein and the influenza virus hemagglutinin are class I viral membrane fusion proteins. While the two proteins display strong structural conservation and the mechanisms underlying membrane fusion are similar, they share no sequence similarity. Whether they are functionally in...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087185/ https://www.ncbi.nlm.nih.gov/pubmed/30136251 http://dx.doi.org/10.1007/s00705-018-4001-9 |
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author | Wanitchang, Asawin Saenboonrueng, Janya Srisutthisamphan, Kanjana Jongkaewwattana, Anan |
author_facet | Wanitchang, Asawin Saenboonrueng, Janya Srisutthisamphan, Kanjana Jongkaewwattana, Anan |
author_sort | Wanitchang, Asawin |
collection | PubMed |
description | The coronavirus spike protein and the influenza virus hemagglutinin are class I viral membrane fusion proteins. While the two proteins display strong structural conservation and the mechanisms underlying membrane fusion are similar, they share no sequence similarity. Whether they are functionally interchangeable is currently unknown. In this study, we constructed scIAV-S, a single-cycle influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus (PEDV), and demonstrated that this virus could infect cultured cells and trigger massive syncytium formation. Treatment with endocytosis inhibitors did not affect syncytium formation by infected cells. Moreover, the infectivity of scIAV-S was associated with the degree of cell adaptation of PEDV-S. Intriguingly, scIAV-S lacking functional neuraminidase (NA) exhibited substantially higher infectivity, suggesting a pivotal role of the sialic acid in the binding/entry of PEDV. Together, scIAV-S offers a robust platform for the investigation of the entry mechanism of PEDV or, possibly, of other coronaviruses. |
format | Online Article Text |
id | pubmed-7087185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-70871852020-03-23 Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus Wanitchang, Asawin Saenboonrueng, Janya Srisutthisamphan, Kanjana Jongkaewwattana, Anan Arch Virol Original Article The coronavirus spike protein and the influenza virus hemagglutinin are class I viral membrane fusion proteins. While the two proteins display strong structural conservation and the mechanisms underlying membrane fusion are similar, they share no sequence similarity. Whether they are functionally interchangeable is currently unknown. In this study, we constructed scIAV-S, a single-cycle influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus (PEDV), and demonstrated that this virus could infect cultured cells and trigger massive syncytium formation. Treatment with endocytosis inhibitors did not affect syncytium formation by infected cells. Moreover, the infectivity of scIAV-S was associated with the degree of cell adaptation of PEDV-S. Intriguingly, scIAV-S lacking functional neuraminidase (NA) exhibited substantially higher infectivity, suggesting a pivotal role of the sialic acid in the binding/entry of PEDV. Together, scIAV-S offers a robust platform for the investigation of the entry mechanism of PEDV or, possibly, of other coronaviruses. Springer Vienna 2018-08-22 2018 /pmc/articles/PMC7087185/ /pubmed/30136251 http://dx.doi.org/10.1007/s00705-018-4001-9 Text en © Springer-Verlag GmbH Austria, part of Springer Nature 2018 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Wanitchang, Asawin Saenboonrueng, Janya Srisutthisamphan, Kanjana Jongkaewwattana, Anan Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus |
title | Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus |
title_full | Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus |
title_fullStr | Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus |
title_full_unstemmed | Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus |
title_short | Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus |
title_sort | characterization of influenza a virus pseudotyped with the spike protein of porcine epidemic diarrhea virus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087185/ https://www.ncbi.nlm.nih.gov/pubmed/30136251 http://dx.doi.org/10.1007/s00705-018-4001-9 |
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