Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA

 In this study feline (FECV and FIPV) and canine (CCoV) coronavirus entry into and release from polarized porcine epithelial LLC-PK1 cells, stably expressing the recombinant feline aminopeptidase-N cDNA, were investigated. Virus entry appeared to occur preferentially through the apical membrane, sim...

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Detalles Bibliográficos
Autores principales: Rossen, J. W. A., Kouame, J., Goedheer, A. J. W., Vennema, H., Rottier, P. J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2001
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087204/
https://www.ncbi.nlm.nih.gov/pubmed/11402864
http://dx.doi.org/10.1007/s007050170147
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author Rossen, J. W. A.
Kouame, J.
Goedheer, A. J. W.
Vennema, H.
Rottier, P. J. M.
author_facet Rossen, J. W. A.
Kouame, J.
Goedheer, A. J. W.
Vennema, H.
Rottier, P. J. M.
author_sort Rossen, J. W. A.
collection PubMed
description  In this study feline (FECV and FIPV) and canine (CCoV) coronavirus entry into and release from polarized porcine epithelial LLC-PK1 cells, stably expressing the recombinant feline aminopeptidase-N cDNA, were investigated. Virus entry appeared to occur preferentially through the apical membrane, similar to the entry of the related porcine coronavirus transmissible gastroenteritis virus (TGEV) into these cells. However, whereas TGEV is released apically, feline and canine coronaviruses were found to be released from the basolateral side of the epithelial cells. These observations indicate that local infections as caused by TGEV, FECV and CCoV do not strictly correlate with apical release, as suggested by earlier work.
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spelling pubmed-70872042020-03-23 Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA Rossen, J. W. A. Kouame, J. Goedheer, A. J. W. Vennema, H. Rottier, P. J. M. Arch Virol Brief Report  In this study feline (FECV and FIPV) and canine (CCoV) coronavirus entry into and release from polarized porcine epithelial LLC-PK1 cells, stably expressing the recombinant feline aminopeptidase-N cDNA, were investigated. Virus entry appeared to occur preferentially through the apical membrane, similar to the entry of the related porcine coronavirus transmissible gastroenteritis virus (TGEV) into these cells. However, whereas TGEV is released apically, feline and canine coronaviruses were found to be released from the basolateral side of the epithelial cells. These observations indicate that local infections as caused by TGEV, FECV and CCoV do not strictly correlate with apical release, as suggested by earlier work. Springer-Verlag 2001 /pmc/articles/PMC7087204/ /pubmed/11402864 http://dx.doi.org/10.1007/s007050170147 Text en © Springer-Verlag/Wien 2001 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Rossen, J. W. A.
Kouame, J.
Goedheer, A. J. W.
Vennema, H.
Rottier, P. J. M.
Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA
title Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA
title_full Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA
title_fullStr Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA
title_full_unstemmed Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA
title_short Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA
title_sort feline and canine coronaviruses are released from the basolateral side of polarized epithelial llc-pk1 cells expressing the recombinant feline aminopeptidase-n cdna
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087204/
https://www.ncbi.nlm.nih.gov/pubmed/11402864
http://dx.doi.org/10.1007/s007050170147
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