In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3

Thein vitro virus yield of MHV3 reached 10(7) PFU/ml in mouse DBT cells infected with a virus suspension in HEPES-buffered medium containing DEAE-dextran. The virus titer was 10(6) PFU/ml in the presence of 10 µg actinomycin D/ml. MHV3 grown in DBT cells gave three peaks of density (1.10–1.14 g/cm(3...

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Detalles Bibliográficos
Autores principales: Takayama, N., Kirn, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1978
Materias:
Original Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087213/
https://www.ncbi.nlm.nih.gov/pubmed/211989
http://dx.doi.org/10.1007/BF01315532
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author Takayama, N.
Kirn, A.
author_facet Takayama, N.
Kirn, A.
author_sort Takayama, N.
collection PubMed
description Thein vitro virus yield of MHV3 reached 10(7) PFU/ml in mouse DBT cells infected with a virus suspension in HEPES-buffered medium containing DEAE-dextran. The virus titer was 10(6) PFU/ml in the presence of 10 µg actinomycin D/ml. MHV3 grown in DBT cells gave three peaks of density (1.10–1.14 g/cm(3), 1.18–1.20 g/cm(3), and 1.25–1.31 g/cm(3)) in sucrose gradients. All these peaks retained infectivity.
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spelling pubmed-70872132020-03-23 In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3 Takayama, N. Kirn, A. Arch Virol Original Papers Thein vitro virus yield of MHV3 reached 10(7) PFU/ml in mouse DBT cells infected with a virus suspension in HEPES-buffered medium containing DEAE-dextran. The virus titer was 10(6) PFU/ml in the presence of 10 µg actinomycin D/ml. MHV3 grown in DBT cells gave three peaks of density (1.10–1.14 g/cm(3), 1.18–1.20 g/cm(3), and 1.25–1.31 g/cm(3)) in sucrose gradients. All these peaks retained infectivity. Springer-Verlag 1978 /pmc/articles/PMC7087213/ /pubmed/211989 http://dx.doi.org/10.1007/BF01315532 Text en © Springer-Verlag 1978 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Papers
Takayama, N.
Kirn, A.
In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3
title In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3
title_full In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3
title_fullStr In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3
title_full_unstemmed In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3
title_short In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3
title_sort in vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087213/
https://www.ncbi.nlm.nih.gov/pubmed/211989
http://dx.doi.org/10.1007/BF01315532
work_keys_str_mv AT takayaman invitrogrowthcharacteristicsandheterogeneityofmousehepatitisvirustype3
AT kirna invitrogrowthcharacteristicsandheterogeneityofmousehepatitisvirustype3

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