Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs

Hepatitis C virus (HCV) is believed to assemble by budding into membranes of the early secretory pathway, consistent with the membrane location where the viral envelope glycoproteins E1 and E2 accumulate when expressed. Coronavirus assembly also takes place at pre-Golgi membranes. Here, we generated...

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Detalles Bibliográficos
Autores principales: Jourdan, N., Godeke, G.-J., Penaud, M., Mottola, G., Sorrentino, A., Rottier, P. J. M., Bonatti, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2006
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087226/
https://www.ncbi.nlm.nih.gov/pubmed/16648962
http://dx.doi.org/10.1007/s00705-006-0769-0
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author Jourdan, N.
Godeke, G.-J.
Penaud, M.
Mottola, G.
Sorrentino, A.
Rottier, P. J. M.
Bonatti, S.
author_facet Jourdan, N.
Godeke, G.-J.
Penaud, M.
Mottola, G.
Sorrentino, A.
Rottier, P. J. M.
Bonatti, S.
author_sort Jourdan, N.
collection PubMed
description Hepatitis C virus (HCV) is believed to assemble by budding into membranes of the early secretory pathway, consistent with the membrane location where the viral envelope glycoproteins E1 and E2 accumulate when expressed. Coronavirus assembly also takes place at pre-Golgi membranes. Here, we generated coronavirus-like particles carrying in their envelope chimeric HCV glycoproteins composed of the ectodomains of E1 and E2, each fused to the transmembrane plus endodomain of the mouse hepatitis coronavirus spike glycoprotein. The chimeric particle system will enable structural and functional studies of the HCV glycoproteins.
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spelling pubmed-70872262020-03-23 Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs Jourdan, N. Godeke, G.-J. Penaud, M. Mottola, G. Sorrentino, A. Rottier, P. J. M. Bonatti, S. Arch Virol Brief Report Hepatitis C virus (HCV) is believed to assemble by budding into membranes of the early secretory pathway, consistent with the membrane location where the viral envelope glycoproteins E1 and E2 accumulate when expressed. Coronavirus assembly also takes place at pre-Golgi membranes. Here, we generated coronavirus-like particles carrying in their envelope chimeric HCV glycoproteins composed of the ectodomains of E1 and E2, each fused to the transmembrane plus endodomain of the mouse hepatitis coronavirus spike glycoprotein. The chimeric particle system will enable structural and functional studies of the HCV glycoproteins. Springer-Verlag 2006-05-03 2006 /pmc/articles/PMC7087226/ /pubmed/16648962 http://dx.doi.org/10.1007/s00705-006-0769-0 Text en © Springer-Verlag 2006 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Jourdan, N.
Godeke, G.-J.
Penaud, M.
Mottola, G.
Sorrentino, A.
Rottier, P. J. M.
Bonatti, S.
Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs
title Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs
title_full Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs
title_fullStr Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs
title_full_unstemmed Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs
title_short Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs
title_sort assembly of hcv e1 and e2 glycoproteins into coronavirus vlps
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087226/
https://www.ncbi.nlm.nih.gov/pubmed/16648962
http://dx.doi.org/10.1007/s00705-006-0769-0
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