Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification

A novel recombinase polymerase amplification (RPA)-based method for detection of canine parvovirus type 2 (CPV-2) was developed. Sensitivity analysis showed that the detection limit of RPA was 10 copies of CPV-2 genomic DNA. RPA amplified both CPV-2a and -2b DNA but did not amplify the template of o...

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Autores principales: Wang, Jianchang, Liu, Libing, Li, Ruiwen, Wang, Jinfeng, Fu, Qi, Yuan, Wanzhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087227/
https://www.ncbi.nlm.nih.gov/pubmed/26729477
http://dx.doi.org/10.1007/s00705-015-2738-y
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author Wang, Jianchang
Liu, Libing
Li, Ruiwen
Wang, Jinfeng
Fu, Qi
Yuan, Wanzhe
author_facet Wang, Jianchang
Liu, Libing
Li, Ruiwen
Wang, Jinfeng
Fu, Qi
Yuan, Wanzhe
author_sort Wang, Jianchang
collection PubMed
description A novel recombinase polymerase amplification (RPA)-based method for detection of canine parvovirus type 2 (CPV-2) was developed. Sensitivity analysis showed that the detection limit of RPA was 10 copies of CPV-2 genomic DNA. RPA amplified both CPV-2a and -2b DNA but did not amplify the template of other important dog viruses (CCoV, PRV or CDV), demonstrating high specificity. The method was further validated with 57 canine fecal samples. An outstanding advantage of RPA is that it is an isothermal reaction and can be performed in a water bath, making RPA a potential alternative method for CPV-2 detection in resource-limited settings.
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spelling pubmed-70872272020-03-23 Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification Wang, Jianchang Liu, Libing Li, Ruiwen Wang, Jinfeng Fu, Qi Yuan, Wanzhe Arch Virol Brief Report A novel recombinase polymerase amplification (RPA)-based method for detection of canine parvovirus type 2 (CPV-2) was developed. Sensitivity analysis showed that the detection limit of RPA was 10 copies of CPV-2 genomic DNA. RPA amplified both CPV-2a and -2b DNA but did not amplify the template of other important dog viruses (CCoV, PRV or CDV), demonstrating high specificity. The method was further validated with 57 canine fecal samples. An outstanding advantage of RPA is that it is an isothermal reaction and can be performed in a water bath, making RPA a potential alternative method for CPV-2 detection in resource-limited settings. Springer Vienna 2016-01-05 2016 /pmc/articles/PMC7087227/ /pubmed/26729477 http://dx.doi.org/10.1007/s00705-015-2738-y Text en © Springer-Verlag Wien 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Wang, Jianchang
Liu, Libing
Li, Ruiwen
Wang, Jinfeng
Fu, Qi
Yuan, Wanzhe
Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
title Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
title_full Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
title_fullStr Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
title_full_unstemmed Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
title_short Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
title_sort rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087227/
https://www.ncbi.nlm.nih.gov/pubmed/26729477
http://dx.doi.org/10.1007/s00705-015-2738-y
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