Cargando…

Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system

Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease causing severe hemorrhagic symptoms with a nearly 30 % case-fatality rate in humans. The experimental use of CCHF virus (CCHFV), which causes CCHF, requires high-biosafety-level (BSL) containment. In contrast, pseudotyping of various vir...

Descripción completa

Detalles Bibliográficos
Autores principales: Suda, Yuto, Fukushi, Shuetsu, Tani, Hideki, Murakami, Shin, Saijo, Masayuki, Horimoto, Taisuke, Shimojima, Masayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087235/
https://www.ncbi.nlm.nih.gov/pubmed/26935918
http://dx.doi.org/10.1007/s00705-016-2803-1
_version_ 1783509296658513920
author Suda, Yuto
Fukushi, Shuetsu
Tani, Hideki
Murakami, Shin
Saijo, Masayuki
Horimoto, Taisuke
Shimojima, Masayuki
author_facet Suda, Yuto
Fukushi, Shuetsu
Tani, Hideki
Murakami, Shin
Saijo, Masayuki
Horimoto, Taisuke
Shimojima, Masayuki
author_sort Suda, Yuto
collection PubMed
description Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease causing severe hemorrhagic symptoms with a nearly 30 % case-fatality rate in humans. The experimental use of CCHF virus (CCHFV), which causes CCHF, requires high-biosafety-level (BSL) containment. In contrast, pseudotyping of various viral glycoproteins (GPs) onto vesicular stomatitis virus (VSV) can be used in facilities with lower BSL containment, and this has facilitated studies on the viral entry mechanism and the measurement of neutralizing activity, especially for highly pathogenic viruses. In the present study, we generated high titers of pseudotyped VSV bearing the CCHFV envelope GP and analyzed the mechanisms involved in CCHFV infection. A partial deletion of the CCHFV GP cytoplasmic domain increased the titer of the pseudotyped VSV, the entry mechanism of which was dependent on the CCHFV envelope GP. Using the pseudotype virus, DC-SIGN (a calcium-dependent [C-type] lectin cell-surface molecule) was revealed to enhance viral infection and act as an entry factor for CCHFV.
format Online
Article
Text
id pubmed-7087235
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-70872352020-03-23 Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system Suda, Yuto Fukushi, Shuetsu Tani, Hideki Murakami, Shin Saijo, Masayuki Horimoto, Taisuke Shimojima, Masayuki Arch Virol Original Article Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease causing severe hemorrhagic symptoms with a nearly 30 % case-fatality rate in humans. The experimental use of CCHF virus (CCHFV), which causes CCHF, requires high-biosafety-level (BSL) containment. In contrast, pseudotyping of various viral glycoproteins (GPs) onto vesicular stomatitis virus (VSV) can be used in facilities with lower BSL containment, and this has facilitated studies on the viral entry mechanism and the measurement of neutralizing activity, especially for highly pathogenic viruses. In the present study, we generated high titers of pseudotyped VSV bearing the CCHFV envelope GP and analyzed the mechanisms involved in CCHFV infection. A partial deletion of the CCHFV GP cytoplasmic domain increased the titer of the pseudotyped VSV, the entry mechanism of which was dependent on the CCHFV envelope GP. Using the pseudotype virus, DC-SIGN (a calcium-dependent [C-type] lectin cell-surface molecule) was revealed to enhance viral infection and act as an entry factor for CCHFV. Springer Vienna 2016-03-03 2016 /pmc/articles/PMC7087235/ /pubmed/26935918 http://dx.doi.org/10.1007/s00705-016-2803-1 Text en © Springer-Verlag Wien 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Suda, Yuto
Fukushi, Shuetsu
Tani, Hideki
Murakami, Shin
Saijo, Masayuki
Horimoto, Taisuke
Shimojima, Masayuki
Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system
title Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system
title_full Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system
title_fullStr Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system
title_full_unstemmed Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system
title_short Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system
title_sort analysis of the entry mechanism of crimean-congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087235/
https://www.ncbi.nlm.nih.gov/pubmed/26935918
http://dx.doi.org/10.1007/s00705-016-2803-1
work_keys_str_mv AT sudayuto analysisoftheentrymechanismofcrimeancongohemorrhagicfevervirususingavesicularstomatitisviruspseudotypingsystem
AT fukushishuetsu analysisoftheentrymechanismofcrimeancongohemorrhagicfevervirususingavesicularstomatitisviruspseudotypingsystem
AT tanihideki analysisoftheentrymechanismofcrimeancongohemorrhagicfevervirususingavesicularstomatitisviruspseudotypingsystem
AT murakamishin analysisoftheentrymechanismofcrimeancongohemorrhagicfevervirususingavesicularstomatitisviruspseudotypingsystem
AT saijomasayuki analysisoftheentrymechanismofcrimeancongohemorrhagicfevervirususingavesicularstomatitisviruspseudotypingsystem
AT horimototaisuke analysisoftheentrymechanismofcrimeancongohemorrhagicfevervirususingavesicularstomatitisviruspseudotypingsystem
AT shimojimamasayuki analysisoftheentrymechanismofcrimeancongohemorrhagicfevervirususingavesicularstomatitisviruspseudotypingsystem