Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro

This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV r...

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Detalles Bibliográficos
Autores principales: Wei, Zhan-Yong, Wang, Xue-Bin, Ning, Xiao-Dong, Wang, Ya-Bin, Zhang, Hong-Ying, Wang, Dong-Fang, Chen, Hong-Ying, Cui, Bao-An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2009
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087247/
https://www.ncbi.nlm.nih.gov/pubmed/19437101
http://dx.doi.org/10.1007/s00705-009-0392-y
Descripción
Sumario:This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME). By assaying the steps of the PPV life cycle, we also show that NO inhibits viral DNA and protein synthesis. This experiment provides a frame of reference for the study of the anti-viral mechanism of NO.

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