Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro

This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV r...

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Autores principales: Wei, Zhan-Yong, Wang, Xue-Bin, Ning, Xiao-Dong, Wang, Ya-Bin, Zhang, Hong-Ying, Wang, Dong-Fang, Chen, Hong-Ying, Cui, Bao-An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2009
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087247/
https://www.ncbi.nlm.nih.gov/pubmed/19437101
http://dx.doi.org/10.1007/s00705-009-0392-y
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author Wei, Zhan-Yong
Wang, Xue-Bin
Ning, Xiao-Dong
Wang, Ya-Bin
Zhang, Hong-Ying
Wang, Dong-Fang
Chen, Hong-Ying
Cui, Bao-An
author_facet Wei, Zhan-Yong
Wang, Xue-Bin
Ning, Xiao-Dong
Wang, Ya-Bin
Zhang, Hong-Ying
Wang, Dong-Fang
Chen, Hong-Ying
Cui, Bao-An
author_sort Wei, Zhan-Yong
collection PubMed
description This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME). By assaying the steps of the PPV life cycle, we also show that NO inhibits viral DNA and protein synthesis. This experiment provides a frame of reference for the study of the anti-viral mechanism of NO.
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spelling pubmed-70872472020-03-23 Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro Wei, Zhan-Yong Wang, Xue-Bin Ning, Xiao-Dong Wang, Ya-Bin Zhang, Hong-Ying Wang, Dong-Fang Chen, Hong-Ying Cui, Bao-An Arch Virol Brief Report This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME). By assaying the steps of the PPV life cycle, we also show that NO inhibits viral DNA and protein synthesis. This experiment provides a frame of reference for the study of the anti-viral mechanism of NO. Springer Vienna 2009-05-13 2009 /pmc/articles/PMC7087247/ /pubmed/19437101 http://dx.doi.org/10.1007/s00705-009-0392-y Text en © Springer-Verlag 2009 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Wei, Zhan-Yong
Wang, Xue-Bin
Ning, Xiao-Dong
Wang, Ya-Bin
Zhang, Hong-Ying
Wang, Dong-Fang
Chen, Hong-Ying
Cui, Bao-An
Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro
title Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro
title_full Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro
title_fullStr Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro
title_full_unstemmed Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro
title_short Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro
title_sort nitric oxide inhibits the replication cycle of porcine parvovirus in vitro
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087247/
https://www.ncbi.nlm.nih.gov/pubmed/19437101
http://dx.doi.org/10.1007/s00705-009-0392-y
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