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Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China
A strain of transmissible gastroenteritis virus (TGEV), SHXB, was isolated in Shanghai, China. The complete genome of strain SHXB was sequenced, and its sequence was compared those of other TGEV strains in the GenBank database. The comparison showed that there were no insertions or deletions in the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087249/ https://www.ncbi.nlm.nih.gov/pubmed/24740387 http://dx.doi.org/10.1007/s00705-014-2080-9 |
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author | Weiwei, Hu Qinghua, Yu Liqi, Zhu Haofei, Liu Shanshan, Zhao Qi, Gao Kongwang, He Qian, Yang |
author_facet | Weiwei, Hu Qinghua, Yu Liqi, Zhu Haofei, Liu Shanshan, Zhao Qi, Gao Kongwang, He Qian, Yang |
author_sort | Weiwei, Hu |
collection | PubMed |
description | A strain of transmissible gastroenteritis virus (TGEV), SHXB, was isolated in Shanghai, China. The complete genome of strain SHXB was sequenced, and its sequence was compared those of other TGEV strains in the GenBank database. The comparison showed that there were no insertions or deletions in the 5′ and 3′- non-translated regions, in the nonstructural genes ORF1, ORF3, and ORF7, or in the genes encoding the structural proteins envelope (E), membrane (M) and nucleoprotein (N). A phenomenon in common with other strains was that nucleotide (nt) 655 of the spike (S) gene was G, and a common change in nt 1753 of the S gene was a T-to-G mutation that caused a serine-to-alanine mutation at amino acid 585, which is in the region of the main major antigenic sites A and B of the TGEV S protein. A 6-nt deletion was also found at nt 1123-1128 in all Purdue strains except the strain Virulent Purdue. Phylogenetic analysis showed that TGEV SHXB was closely related to the Purdue strains and shared a common ancestor with the Miller strains as well as strain PRCV-ISU-1. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-014-2080-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7087249 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-70872492020-03-23 Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China Weiwei, Hu Qinghua, Yu Liqi, Zhu Haofei, Liu Shanshan, Zhao Qi, Gao Kongwang, He Qian, Yang Arch Virol Original Article A strain of transmissible gastroenteritis virus (TGEV), SHXB, was isolated in Shanghai, China. The complete genome of strain SHXB was sequenced, and its sequence was compared those of other TGEV strains in the GenBank database. The comparison showed that there were no insertions or deletions in the 5′ and 3′- non-translated regions, in the nonstructural genes ORF1, ORF3, and ORF7, or in the genes encoding the structural proteins envelope (E), membrane (M) and nucleoprotein (N). A phenomenon in common with other strains was that nucleotide (nt) 655 of the spike (S) gene was G, and a common change in nt 1753 of the S gene was a T-to-G mutation that caused a serine-to-alanine mutation at amino acid 585, which is in the region of the main major antigenic sites A and B of the TGEV S protein. A 6-nt deletion was also found at nt 1123-1128 in all Purdue strains except the strain Virulent Purdue. Phylogenetic analysis showed that TGEV SHXB was closely related to the Purdue strains and shared a common ancestor with the Miller strains as well as strain PRCV-ISU-1. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-014-2080-9) contains supplementary material, which is available to authorized users. Springer Vienna 2014-04-17 2014 /pmc/articles/PMC7087249/ /pubmed/24740387 http://dx.doi.org/10.1007/s00705-014-2080-9 Text en © Springer-Verlag Wien 2014 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Weiwei, Hu Qinghua, Yu Liqi, Zhu Haofei, Liu Shanshan, Zhao Qi, Gao Kongwang, He Qian, Yang Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China |
title | Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China |
title_full | Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China |
title_fullStr | Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China |
title_full_unstemmed | Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China |
title_short | Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China |
title_sort | complete genomic sequence of the coronavirus transmissible gastroenteritis virus shxb isolated in china |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087249/ https://www.ncbi.nlm.nih.gov/pubmed/24740387 http://dx.doi.org/10.1007/s00705-014-2080-9 |
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