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An astrovirus frameshift signal induces ribosomal frameshifting in vitro

Expression of the astrovirus RNA-dependent RNA polymerase has been hypothesized to be regulated by (− 1) ribosomal frameshifting. Sequence analysis of the 70 nucleotide region between open reading frames 1a and 1b indicates the presence of a shifty heptamer consensus sequence and downstream sequence...

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Detalles Bibliográficos
Autores principales: Lewis, T. L., Matsui, S. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087256/
https://www.ncbi.nlm.nih.gov/pubmed/7611883
http://dx.doi.org/10.1007/BF01315421
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author Lewis, T. L.
Matsui, S. M.
author_facet Lewis, T. L.
Matsui, S. M.
author_sort Lewis, T. L.
collection PubMed
description Expression of the astrovirus RNA-dependent RNA polymerase has been hypothesized to be regulated by (− 1) ribosomal frameshifting. Sequence analysis of the 70 nucleotide region between open reading frames 1a and 1b indicates the presence of a shifty heptamer consensus sequence and downstream sequences that may be needed for ribosomal frameshifting. We constructed four astrovirus cassettes that spanned this region and inserted each into the rhesus rotavirus VP4 gene. The constructs were expressed in an in vitro system, and products were immunoprecipitated by rotavirus amino and carboxy terminal-specific monoclonal antibodies. Ribosomal frameshifting, at an efficiency of 6–7%, was demonstrated in all constructs containing the shifty heptamer and stem-loop. Deletion of the downstream sequence potentially involved in pseudoknot formation did not affect frameshifting efficiency. However, deletion of the shifty heptamer resulted in no detectable frameshift activity.
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spelling pubmed-70872562020-03-23 An astrovirus frameshift signal induces ribosomal frameshifting in vitro Lewis, T. L. Matsui, S. M. Arch Virol Brief Report Expression of the astrovirus RNA-dependent RNA polymerase has been hypothesized to be regulated by (− 1) ribosomal frameshifting. Sequence analysis of the 70 nucleotide region between open reading frames 1a and 1b indicates the presence of a shifty heptamer consensus sequence and downstream sequences that may be needed for ribosomal frameshifting. We constructed four astrovirus cassettes that spanned this region and inserted each into the rhesus rotavirus VP4 gene. The constructs were expressed in an in vitro system, and products were immunoprecipitated by rotavirus amino and carboxy terminal-specific monoclonal antibodies. Ribosomal frameshifting, at an efficiency of 6–7%, was demonstrated in all constructs containing the shifty heptamer and stem-loop. Deletion of the downstream sequence potentially involved in pseudoknot formation did not affect frameshifting efficiency. However, deletion of the shifty heptamer resulted in no detectable frameshift activity. Springer-Verlag 1995 /pmc/articles/PMC7087256/ /pubmed/7611883 http://dx.doi.org/10.1007/BF01315421 Text en © Springer-Verlag 1995 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Lewis, T. L.
Matsui, S. M.
An astrovirus frameshift signal induces ribosomal frameshifting in vitro
title An astrovirus frameshift signal induces ribosomal frameshifting in vitro
title_full An astrovirus frameshift signal induces ribosomal frameshifting in vitro
title_fullStr An astrovirus frameshift signal induces ribosomal frameshifting in vitro
title_full_unstemmed An astrovirus frameshift signal induces ribosomal frameshifting in vitro
title_short An astrovirus frameshift signal induces ribosomal frameshifting in vitro
title_sort astrovirus frameshift signal induces ribosomal frameshifting in vitro
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087256/
https://www.ncbi.nlm.nih.gov/pubmed/7611883
http://dx.doi.org/10.1007/BF01315421
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