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Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay

An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP,...

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Detalles Bibliográficos
Autores principales: Yang, Jin, Fang, Mei-xin, Li, Jie, Lou, Guo-qiang, Lu, Hang-jun, Wu, Nan-ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087303/
https://www.ncbi.nlm.nih.gov/pubmed/21562883
http://dx.doi.org/10.1007/s00705-011-1001-4
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author Yang, Jin
Fang, Mei-xin
Li, Jie
Lou, Guo-qiang
Lu, Hang-jun
Wu, Nan-ping
author_facet Yang, Jin
Fang, Mei-xin
Li, Jie
Lou, Guo-qiang
Lu, Hang-jun
Wu, Nan-ping
author_sort Yang, Jin
collection PubMed
description An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.
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spelling pubmed-70873032020-03-23 Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay Yang, Jin Fang, Mei-xin Li, Jie Lou, Guo-qiang Lu, Hang-jun Wu, Nan-ping Arch Virol Original Article An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA. Springer Vienna 2011-05-12 2011 /pmc/articles/PMC7087303/ /pubmed/21562883 http://dx.doi.org/10.1007/s00705-011-1001-4 Text en © Springer-Verlag 2011 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Yang, Jin
Fang, Mei-xin
Li, Jie
Lou, Guo-qiang
Lu, Hang-jun
Wu, Nan-ping
Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
title Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
title_full Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
title_fullStr Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
title_full_unstemmed Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
title_short Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
title_sort detection of hepatitis c virus by an improved loop-mediated isothermal amplification assay
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087303/
https://www.ncbi.nlm.nih.gov/pubmed/21562883
http://dx.doi.org/10.1007/s00705-011-1001-4
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