Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans

We previously reported that cells with persistent severe acute respiratory syndrome coronavirus (SARS-CoV) infection were established after apoptotic events. In the present study, we investigated the cytopathic effects of dual infection with SARS-CoV and Mycoplasma fermentans on Vero E6 cells. Dual...

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Autores principales: Mizutani, T., Fukushi, S., Kenri, T., Sasaki, Y., Ishii, K., Endoh, D., Zamoto, A., Saijo, M., Kurane, I., Morikawa, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2007
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087332/
https://www.ncbi.nlm.nih.gov/pubmed/17277901
http://dx.doi.org/10.1007/s00705-006-0924-7
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author Mizutani, T.
Fukushi, S.
Kenri, T.
Sasaki, Y.
Ishii, K.
Endoh, D.
Zamoto, A.
Saijo, M.
Kurane, I.
Morikawa, S.
author_facet Mizutani, T.
Fukushi, S.
Kenri, T.
Sasaki, Y.
Ishii, K.
Endoh, D.
Zamoto, A.
Saijo, M.
Kurane, I.
Morikawa, S.
author_sort Mizutani, T.
collection PubMed
description We previously reported that cells with persistent severe acute respiratory syndrome coronavirus (SARS-CoV) infection were established after apoptotic events. In the present study, we investigated the cytopathic effects of dual infection with SARS-CoV and Mycoplasma fermentans on Vero E6 cells. Dual infection completely killed cells and prevented the establishment of persistent SARS-CoV infection. M. fermentans induced inhibition of cell proliferation, but the cells remained alive. Apoptosis was induced easily in M. fermentans-infected cells, indicating that they were primed for apoptosis. These results indicated that M. fermentans enhances apoptosis in surviving cells that have escaped from SARS-CoV-induced apoptosis.
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spelling pubmed-70873322020-03-23 Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans Mizutani, T. Fukushi, S. Kenri, T. Sasaki, Y. Ishii, K. Endoh, D. Zamoto, A. Saijo, M. Kurane, I. Morikawa, S. Arch Virol Brief Report We previously reported that cells with persistent severe acute respiratory syndrome coronavirus (SARS-CoV) infection were established after apoptotic events. In the present study, we investigated the cytopathic effects of dual infection with SARS-CoV and Mycoplasma fermentans on Vero E6 cells. Dual infection completely killed cells and prevented the establishment of persistent SARS-CoV infection. M. fermentans induced inhibition of cell proliferation, but the cells remained alive. Apoptosis was induced easily in M. fermentans-infected cells, indicating that they were primed for apoptosis. These results indicated that M. fermentans enhances apoptosis in surviving cells that have escaped from SARS-CoV-induced apoptosis. Springer-Verlag 2007-02-07 2007 /pmc/articles/PMC7087332/ /pubmed/17277901 http://dx.doi.org/10.1007/s00705-006-0924-7 Text en © Springer-Verlag 2007 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Mizutani, T.
Fukushi, S.
Kenri, T.
Sasaki, Y.
Ishii, K.
Endoh, D.
Zamoto, A.
Saijo, M.
Kurane, I.
Morikawa, S.
Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans
title Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans
title_full Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans
title_fullStr Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans
title_full_unstemmed Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans
title_short Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans
title_sort enhancement of cytotoxicity against vero e6 cells persistently infected with sars-cov by mycoplasma fermentans
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087332/
https://www.ncbi.nlm.nih.gov/pubmed/17277901
http://dx.doi.org/10.1007/s00705-006-0924-7
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