Inhibition of hepatitis E virus replication by proteasome inhibitor is nonspecific

The ubiquitin proteasome system plays important role in virus infection. A previous study showed that the proteasome inhibitor MG132 could potentially affect hepatitis E virus (HEV) replication. In this study, we found that MG132 could inhibit HEV and hepatitis C virus (HCV) replication-related luci...

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Detalles Bibliográficos
Autores principales: Xu, Lei, Zhou, Xinying, Peppelenbosch, Maikel P., Pan, Qiuwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2014
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087333/
https://www.ncbi.nlm.nih.gov/pubmed/25476751
http://dx.doi.org/10.1007/s00705-014-2303-0
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author Xu, Lei
Zhou, Xinying
Peppelenbosch, Maikel P.
Pan, Qiuwei
author_facet Xu, Lei
Zhou, Xinying
Peppelenbosch, Maikel P.
Pan, Qiuwei
author_sort Xu, Lei
collection PubMed
description The ubiquitin proteasome system plays important role in virus infection. A previous study showed that the proteasome inhibitor MG132 could potentially affect hepatitis E virus (HEV) replication. In this study, we found that MG132 could inhibit HEV and hepatitis C virus (HCV) replication-related luciferase activity in subgenomic models. Furthermore, treatment with MG132 in a HEV infectious model resulted in a dramatic reduction in the intracellular level of HEV RNA. Surprisingly, MG132 concurrently inhibited the expression of a luciferase gene used as a control as well as a wide range of host genes. Consistently, the total cellular RNA and protein content was concurrently reduced by MG132 treatment, suggesting a nonspecific antiviral effect.
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spelling pubmed-70873332020-03-23 Inhibition of hepatitis E virus replication by proteasome inhibitor is nonspecific Xu, Lei Zhou, Xinying Peppelenbosch, Maikel P. Pan, Qiuwei Arch Virol Brief Report The ubiquitin proteasome system plays important role in virus infection. A previous study showed that the proteasome inhibitor MG132 could potentially affect hepatitis E virus (HEV) replication. In this study, we found that MG132 could inhibit HEV and hepatitis C virus (HCV) replication-related luciferase activity in subgenomic models. Furthermore, treatment with MG132 in a HEV infectious model resulted in a dramatic reduction in the intracellular level of HEV RNA. Surprisingly, MG132 concurrently inhibited the expression of a luciferase gene used as a control as well as a wide range of host genes. Consistently, the total cellular RNA and protein content was concurrently reduced by MG132 treatment, suggesting a nonspecific antiviral effect. Springer Vienna 2014-12-05 2015 /pmc/articles/PMC7087333/ /pubmed/25476751 http://dx.doi.org/10.1007/s00705-014-2303-0 Text en © Springer-Verlag Wien 2014 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Xu, Lei
Zhou, Xinying
Peppelenbosch, Maikel P.
Pan, Qiuwei
Inhibition of hepatitis E virus replication by proteasome inhibitor is nonspecific
title Inhibition of hepatitis E virus replication by proteasome inhibitor is nonspecific
title_full Inhibition of hepatitis E virus replication by proteasome inhibitor is nonspecific
title_fullStr Inhibition of hepatitis E virus replication by proteasome inhibitor is nonspecific
title_full_unstemmed Inhibition of hepatitis E virus replication by proteasome inhibitor is nonspecific
title_short Inhibition of hepatitis E virus replication by proteasome inhibitor is nonspecific
title_sort inhibition of hepatitis e virus replication by proteasome inhibitor is nonspecific
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087333/
https://www.ncbi.nlm.nih.gov/pubmed/25476751
http://dx.doi.org/10.1007/s00705-014-2303-0
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AT zhouxinying inhibitionofhepatitisevirusreplicationbyproteasomeinhibitorisnonspecific
AT peppelenboschmaikelp inhibitionofhepatitisevirusreplicationbyproteasomeinhibitorisnonspecific
AT panqiuwei inhibitionofhepatitisevirusreplicationbyproteasomeinhibitorisnonspecific

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