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Genomic characterization of circoviruses associated with acute gastroenteritis in minks in northeastern China
Mink circovirus (MiCV), a virus that was newly discovered in 2013, has been associated with enteric disease. However, its etiological role in acute gastroenteritis is unclear, and its genetic characteristics are poorly described. In this study, the role of circoviruses (CVs) in mink acute gastroente...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Vienna
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087342/ https://www.ncbi.nlm.nih.gov/pubmed/29948383 http://dx.doi.org/10.1007/s00705-018-3908-5 |
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author | Ge, Junwei Gu, Shanshan Cui, Xingyang Zhao, Lili Ma, Dexing Shi, Yunjia Wang, Yuanzhi Lu, Taofeng Chen, Hongyan |
author_facet | Ge, Junwei Gu, Shanshan Cui, Xingyang Zhao, Lili Ma, Dexing Shi, Yunjia Wang, Yuanzhi Lu, Taofeng Chen, Hongyan |
author_sort | Ge, Junwei |
collection | PubMed |
description | Mink circovirus (MiCV), a virus that was newly discovered in 2013, has been associated with enteric disease. However, its etiological role in acute gastroenteritis is unclear, and its genetic characteristics are poorly described. In this study, the role of circoviruses (CVs) in mink acute gastroenteritis was investigated, and the MiCV genome was molecularly characterized through sequence analysis. Detection results demonstrated that MiCV was the only pathogen found in this infection. MiCVs and previously characterized CVs shared genome organizational features, including the presence of (i) a potential stem-loop/nonanucleotide motif that is considered to be the origin of virus DNA replication; (ii) two major inversely arranged open reading frames encoding putative replication-associated proteins (Rep) and a capsid protein; (iii) direct and inverse repeated sequences within the putative 5ʹ region; and (iv) motifs in Rep. Pairwise comparisons showed that the capsid proteins of MiCV shared the highest amino acid sequence identity with those of porcine CV (PCV) 2 (45.4%) and bat CV (BatCV) 1 (45.4%). The amino acid sequence identity levels of Rep shared by MiCV with BatCV 1 (79.7%) and dog CV (dogCV) (54.5%) were broadly similar to those with starling CV (51.1%) and PCVs (46.5%). Phylogenetic analysis indicated that MiCVs were more closely related to mammalian CVs, such as BatCV, PCV, and dogCV, than to other animal CVs. Among mammalian CVs, MiCV and BatCV 1 were the most closely related. This study could contribute to understanding the potential pathogenicity of MiCV and the evolutionary and pathogenic characteristics of mammalian CVs. |
format | Online Article Text |
id | pubmed-7087342 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-70873422020-03-23 Genomic characterization of circoviruses associated with acute gastroenteritis in minks in northeastern China Ge, Junwei Gu, Shanshan Cui, Xingyang Zhao, Lili Ma, Dexing Shi, Yunjia Wang, Yuanzhi Lu, Taofeng Chen, Hongyan Arch Virol Original Article Mink circovirus (MiCV), a virus that was newly discovered in 2013, has been associated with enteric disease. However, its etiological role in acute gastroenteritis is unclear, and its genetic characteristics are poorly described. In this study, the role of circoviruses (CVs) in mink acute gastroenteritis was investigated, and the MiCV genome was molecularly characterized through sequence analysis. Detection results demonstrated that MiCV was the only pathogen found in this infection. MiCVs and previously characterized CVs shared genome organizational features, including the presence of (i) a potential stem-loop/nonanucleotide motif that is considered to be the origin of virus DNA replication; (ii) two major inversely arranged open reading frames encoding putative replication-associated proteins (Rep) and a capsid protein; (iii) direct and inverse repeated sequences within the putative 5ʹ region; and (iv) motifs in Rep. Pairwise comparisons showed that the capsid proteins of MiCV shared the highest amino acid sequence identity with those of porcine CV (PCV) 2 (45.4%) and bat CV (BatCV) 1 (45.4%). The amino acid sequence identity levels of Rep shared by MiCV with BatCV 1 (79.7%) and dog CV (dogCV) (54.5%) were broadly similar to those with starling CV (51.1%) and PCVs (46.5%). Phylogenetic analysis indicated that MiCVs were more closely related to mammalian CVs, such as BatCV, PCV, and dogCV, than to other animal CVs. Among mammalian CVs, MiCV and BatCV 1 were the most closely related. This study could contribute to understanding the potential pathogenicity of MiCV and the evolutionary and pathogenic characteristics of mammalian CVs. Springer Vienna 2018-06-14 2018 /pmc/articles/PMC7087342/ /pubmed/29948383 http://dx.doi.org/10.1007/s00705-018-3908-5 Text en © Springer-Verlag GmbH Austria, part of Springer Nature 2018 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Ge, Junwei Gu, Shanshan Cui, Xingyang Zhao, Lili Ma, Dexing Shi, Yunjia Wang, Yuanzhi Lu, Taofeng Chen, Hongyan Genomic characterization of circoviruses associated with acute gastroenteritis in minks in northeastern China |
title | Genomic characterization of circoviruses associated with acute gastroenteritis in minks in northeastern China |
title_full | Genomic characterization of circoviruses associated with acute gastroenteritis in minks in northeastern China |
title_fullStr | Genomic characterization of circoviruses associated with acute gastroenteritis in minks in northeastern China |
title_full_unstemmed | Genomic characterization of circoviruses associated with acute gastroenteritis in minks in northeastern China |
title_short | Genomic characterization of circoviruses associated with acute gastroenteritis in minks in northeastern China |
title_sort | genomic characterization of circoviruses associated with acute gastroenteritis in minks in northeastern china |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087342/ https://www.ncbi.nlm.nih.gov/pubmed/29948383 http://dx.doi.org/10.1007/s00705-018-3908-5 |
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