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MouseV(k) gene classification by nucleic acid sequence similarity

Analyses of immunoglobulin (Ig) variable (V) region gene usage in the immune response, estimates ofV gene germline complexity, and other nucleic acid hybridization-based studies depend on the extent to which such genes are related (i. e., sequence similarity) and their organization in gene families....

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Detalles Bibliográficos
Autores principales: Strohal, Robert, Helmberg, Arno, Kroemer, Guido, Kofler, Reinhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087523/
https://www.ncbi.nlm.nih.gov/pubmed/2574159
http://dx.doi.org/10.1007/BF02421180
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author Strohal, Robert
Helmberg, Arno
Kroemer, Guido
Kofler, Reinhard
author_facet Strohal, Robert
Helmberg, Arno
Kroemer, Guido
Kofler, Reinhard
author_sort Strohal, Robert
collection PubMed
description Analyses of immunoglobulin (Ig) variable (V) region gene usage in the immune response, estimates ofV gene germline complexity, and other nucleic acid hybridization-based studies depend on the extent to which such genes are related (i. e., sequence similarity) and their organization in gene families. While mouseIgh heavy chainV region (V (H)) gene families are relatively well-established, a corresponding systematic classification ofIgk light chainV region (V (k)) genes has not been reported. The present analysis, in the course of which we reviewed the known extent of theV (k) germline gene repertoire andV (k) gene usage in a variety of responses to foreign and self antigens, provides a classification of mouseV (k) genes in gene families composed of members with >80% overall nucleic acid sequence similarity. This classification differed in several aspects from that ofV (H) genes: only someV (k) gene families were as clearly separated (by >25% sequence dissimilarity) as typicalV (H) gene families; mostV (k) gene families were closely related and, in several instances, members from different families were very similar (>80%) over large sequence portions; frequently, classification by nucleic acid sequence similarity diverged from existing classifications based on amino-terminal protein sequence similarity. Our data have implications forV (k) gene analyses by nucleic acid hybridization and describe potentially important differences in sequence organization betweenV (H) andV (k) genes.
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spelling pubmed-70875232020-03-23 MouseV(k) gene classification by nucleic acid sequence similarity Strohal, Robert Helmberg, Arno Kroemer, Guido Kofler, Reinhard Immunogenetics Article Analyses of immunoglobulin (Ig) variable (V) region gene usage in the immune response, estimates ofV gene germline complexity, and other nucleic acid hybridization-based studies depend on the extent to which such genes are related (i. e., sequence similarity) and their organization in gene families. While mouseIgh heavy chainV region (V (H)) gene families are relatively well-established, a corresponding systematic classification ofIgk light chainV region (V (k)) genes has not been reported. The present analysis, in the course of which we reviewed the known extent of theV (k) germline gene repertoire andV (k) gene usage in a variety of responses to foreign and self antigens, provides a classification of mouseV (k) genes in gene families composed of members with >80% overall nucleic acid sequence similarity. This classification differed in several aspects from that ofV (H) genes: only someV (k) gene families were as clearly separated (by >25% sequence dissimilarity) as typicalV (H) gene families; mostV (k) gene families were closely related and, in several instances, members from different families were very similar (>80%) over large sequence portions; frequently, classification by nucleic acid sequence similarity diverged from existing classifications based on amino-terminal protein sequence similarity. Our data have implications forV (k) gene analyses by nucleic acid hybridization and describe potentially important differences in sequence organization betweenV (H) andV (k) genes. Springer-Verlag 1989 /pmc/articles/PMC7087523/ /pubmed/2574159 http://dx.doi.org/10.1007/BF02421180 Text en © Springer-Verlag 1989 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Strohal, Robert
Helmberg, Arno
Kroemer, Guido
Kofler, Reinhard
MouseV(k) gene classification by nucleic acid sequence similarity
title MouseV(k) gene classification by nucleic acid sequence similarity
title_full MouseV(k) gene classification by nucleic acid sequence similarity
title_fullStr MouseV(k) gene classification by nucleic acid sequence similarity
title_full_unstemmed MouseV(k) gene classification by nucleic acid sequence similarity
title_short MouseV(k) gene classification by nucleic acid sequence similarity
title_sort mousev(k) gene classification by nucleic acid sequence similarity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087523/
https://www.ncbi.nlm.nih.gov/pubmed/2574159
http://dx.doi.org/10.1007/BF02421180
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