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Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study)

Symptoms of acute febrile respiratory tract infection are often unspecific, but the rapid identification of pathogens allows optimised patient management. The objective of this study was to evaluate a novel multiplex polymerase chain reaction (PCR) suspension microarray which detects 19 viral and fo...

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Autores principales: Bierbaum, S., Königsfeld, N., Besazza, N., Blessing, K., Rücker, G., Kontny, U., Berner, R., Schumacher, M., Forster, J., Falcone, V., van de Sand, C., Essig, A., Huzly, D., Rohde, G., Neumann-Haefelin, D., Panning, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087590/
https://www.ncbi.nlm.nih.gov/pubmed/22644053
http://dx.doi.org/10.1007/s10096-012-1639-1
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author Bierbaum, S.
Königsfeld, N.
Besazza, N.
Blessing, K.
Rücker, G.
Kontny, U.
Berner, R.
Schumacher, M.
Forster, J.
Falcone, V.
van de Sand, C.
Essig, A.
Huzly, D.
Rohde, G.
Neumann-Haefelin, D.
Panning, M.
author_facet Bierbaum, S.
Königsfeld, N.
Besazza, N.
Blessing, K.
Rücker, G.
Kontny, U.
Berner, R.
Schumacher, M.
Forster, J.
Falcone, V.
van de Sand, C.
Essig, A.
Huzly, D.
Rohde, G.
Neumann-Haefelin, D.
Panning, M.
author_sort Bierbaum, S.
collection PubMed
description Symptoms of acute febrile respiratory tract infection are often unspecific, but the rapid identification of pathogens allows optimised patient management. The objective of this study was to evaluate a novel multiplex polymerase chain reaction (PCR) suspension microarray which detects 19 viral and four atypical bacterial targets. A comprehensive set of sensitive monoplex real-time PCR assays was used for each pathogen as the gold standard. A panel of archived as well as 300 prospectively collected clinical samples was analysed by both methods. At least one target was detected in 165/300 (55 %) samples by monoplex PCR and in 140/300 (46 %) samples by multiplex PCR, respectively. The positivity rate was significantly higher in paediatric patients compared to adults [126/154 (82 %) vs. 39/146 (27 %) by monoplex and 114/154 (74 %) vs. 26/146 (18 %) by multiplex PCR, respectively]. Among all samples, 17/300 (5.6 %) were positive for atypical bacteria by monoplex and 8/300 (2.6 %) by multiplex PCR, respectively. Multiple detections were recorded in 35/300 (11.6 %) samples by monoplex and 26/300 (8.7 %) by multiplex PCR. For the most common pathogens, the sensitivity ranged from 57 to 93 % and the specificity ranged from 95 to 100 %. The overall concordance between both methods was 77 % [95 % confidence interval (CI) 72–81 %]. False-negative results by multiplex PCR were mainly due to the low target concentration. Compared to monoplex PCR, the novel microarray assay proved its principle but displayed overall lower sensitivities, potentially restricting its use to paediatric patients. For some targets, only small numbers of positive samples were available, requiring larger studies to firmly assess the sensitivity and specificity.
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spelling pubmed-70875902020-03-23 Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study) Bierbaum, S. Königsfeld, N. Besazza, N. Blessing, K. Rücker, G. Kontny, U. Berner, R. Schumacher, M. Forster, J. Falcone, V. van de Sand, C. Essig, A. Huzly, D. Rohde, G. Neumann-Haefelin, D. Panning, M. Eur J Clin Microbiol Infect Dis Article Symptoms of acute febrile respiratory tract infection are often unspecific, but the rapid identification of pathogens allows optimised patient management. The objective of this study was to evaluate a novel multiplex polymerase chain reaction (PCR) suspension microarray which detects 19 viral and four atypical bacterial targets. A comprehensive set of sensitive monoplex real-time PCR assays was used for each pathogen as the gold standard. A panel of archived as well as 300 prospectively collected clinical samples was analysed by both methods. At least one target was detected in 165/300 (55 %) samples by monoplex PCR and in 140/300 (46 %) samples by multiplex PCR, respectively. The positivity rate was significantly higher in paediatric patients compared to adults [126/154 (82 %) vs. 39/146 (27 %) by monoplex and 114/154 (74 %) vs. 26/146 (18 %) by multiplex PCR, respectively]. Among all samples, 17/300 (5.6 %) were positive for atypical bacteria by monoplex and 8/300 (2.6 %) by multiplex PCR, respectively. Multiple detections were recorded in 35/300 (11.6 %) samples by monoplex and 26/300 (8.7 %) by multiplex PCR. For the most common pathogens, the sensitivity ranged from 57 to 93 % and the specificity ranged from 95 to 100 %. The overall concordance between both methods was 77 % [95 % confidence interval (CI) 72–81 %]. False-negative results by multiplex PCR were mainly due to the low target concentration. Compared to monoplex PCR, the novel microarray assay proved its principle but displayed overall lower sensitivities, potentially restricting its use to paediatric patients. For some targets, only small numbers of positive samples were available, requiring larger studies to firmly assess the sensitivity and specificity. Springer-Verlag 2012-05-30 2012 /pmc/articles/PMC7087590/ /pubmed/22644053 http://dx.doi.org/10.1007/s10096-012-1639-1 Text en © Springer-Verlag 2012 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Bierbaum, S.
Königsfeld, N.
Besazza, N.
Blessing, K.
Rücker, G.
Kontny, U.
Berner, R.
Schumacher, M.
Forster, J.
Falcone, V.
van de Sand, C.
Essig, A.
Huzly, D.
Rohde, G.
Neumann-Haefelin, D.
Panning, M.
Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study)
title Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study)
title_full Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study)
title_fullStr Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study)
title_full_unstemmed Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study)
title_short Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study)
title_sort performance of a novel microarray multiplex pcr for the detection of 23 respiratory pathogens (symp-ari study)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087590/
https://www.ncbi.nlm.nih.gov/pubmed/22644053
http://dx.doi.org/10.1007/s10096-012-1639-1
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