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Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.)
In some regions, little is known about exposure to viruses in coastal marine mammals. The present study aimed to detect viral RNA or DNA in 23 free-ranging fur seals on the northern coastline of Rio Grande do Sul State, Brazil. Polymerase chain reaction was used to detect nucleic acids of circovirus...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087719/ https://www.ncbi.nlm.nih.gov/pubmed/27803979 http://dx.doi.org/10.1007/s10393-016-1195-8 |
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author | Chiappetta, Catarina Marcon Cibulski, Samuel Paulo Lima, Francisco Esmaile Sales Varela, Ana Paula Muterle Amorim, Derek Blaese Tavares, Maurício Roehe, Paulo Michel |
author_facet | Chiappetta, Catarina Marcon Cibulski, Samuel Paulo Lima, Francisco Esmaile Sales Varela, Ana Paula Muterle Amorim, Derek Blaese Tavares, Maurício Roehe, Paulo Michel |
author_sort | Chiappetta, Catarina Marcon |
collection | PubMed |
description | In some regions, little is known about exposure to viruses in coastal marine mammals. The present study aimed to detect viral RNA or DNA in 23 free-ranging fur seals on the northern coastline of Rio Grande do Sul State, Brazil. Polymerase chain reaction was used to detect nucleic acids of circoviruses, adenoviruses, morbilliviruses, vesiviruses, and coronaviruses in the feces from twenty-one South American fur seals (Arctocephalus australis) and two Subantarctic fur seals (A. tropicalis). Adenovirus DNA fragments were detected in two South American fur seals; nucleotide sequences of these fragments revealed a high degree of similarity to human adenovirus type C. Circovirus DNA fragments were detected in six animals of the same species. Two were phylogenetically similar to the Circovirus genus, whereas the other four nucleotide fragments showed no similarity to any of the known genera within the family Circoviridae. RNA fragments indicating the presence of coronavirus, vesivirus, and morbillivirus were not detected. These findings suggest that adenoviruses and circoviruses are circulating in fur seal populations found along the coast of Rio Grande do Sul State, Brazil. |
format | Online Article Text |
id | pubmed-7087719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-70877192020-03-23 Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.) Chiappetta, Catarina Marcon Cibulski, Samuel Paulo Lima, Francisco Esmaile Sales Varela, Ana Paula Muterle Amorim, Derek Blaese Tavares, Maurício Roehe, Paulo Michel Ecohealth Original Contribution In some regions, little is known about exposure to viruses in coastal marine mammals. The present study aimed to detect viral RNA or DNA in 23 free-ranging fur seals on the northern coastline of Rio Grande do Sul State, Brazil. Polymerase chain reaction was used to detect nucleic acids of circoviruses, adenoviruses, morbilliviruses, vesiviruses, and coronaviruses in the feces from twenty-one South American fur seals (Arctocephalus australis) and two Subantarctic fur seals (A. tropicalis). Adenovirus DNA fragments were detected in two South American fur seals; nucleotide sequences of these fragments revealed a high degree of similarity to human adenovirus type C. Circovirus DNA fragments were detected in six animals of the same species. Two were phylogenetically similar to the Circovirus genus, whereas the other four nucleotide fragments showed no similarity to any of the known genera within the family Circoviridae. RNA fragments indicating the presence of coronavirus, vesivirus, and morbillivirus were not detected. These findings suggest that adenoviruses and circoviruses are circulating in fur seal populations found along the coast of Rio Grande do Sul State, Brazil. Springer US 2016-11-01 2017 /pmc/articles/PMC7087719/ /pubmed/27803979 http://dx.doi.org/10.1007/s10393-016-1195-8 Text en © International Association for Ecology and Health 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Contribution Chiappetta, Catarina Marcon Cibulski, Samuel Paulo Lima, Francisco Esmaile Sales Varela, Ana Paula Muterle Amorim, Derek Blaese Tavares, Maurício Roehe, Paulo Michel Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.) |
title | Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.) |
title_full | Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.) |
title_fullStr | Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.) |
title_full_unstemmed | Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.) |
title_short | Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.) |
title_sort | molecular detection of circovirus and adenovirus in feces of fur seals (arctocephalus spp.) |
topic | Original Contribution |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087719/ https://www.ncbi.nlm.nih.gov/pubmed/27803979 http://dx.doi.org/10.1007/s10393-016-1195-8 |
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