Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli

Seven overlapping truncated forms of the C subunit of porcine aminopeptidase N (pAPN-C) were expressed in Escherichia coli. By western blotting and ELISA test, all recombinant proteins were recognized by the antibody against native porcine aminopeptidase N. Recombinant proteins, rpAPN-C2 (aa 623–722...

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Autores principales: Sun, Dongbo, Shi, Hongyan, Chen, Jianfei, Guo, Donghua, Liu, Quan, He, Xianjing, Bao, Jun, Wang, Yunfeng, Qiu, Huaji, Feng, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087752/
https://www.ncbi.nlm.nih.gov/pubmed/22083718
http://dx.doi.org/10.1007/s10529-011-0795-1
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author Sun, Dongbo
Shi, Hongyan
Chen, Jianfei
Guo, Donghua
Liu, Quan
He, Xianjing
Bao, Jun
Wang, Yunfeng
Qiu, Huaji
Feng, Li
author_facet Sun, Dongbo
Shi, Hongyan
Chen, Jianfei
Guo, Donghua
Liu, Quan
He, Xianjing
Bao, Jun
Wang, Yunfeng
Qiu, Huaji
Feng, Li
author_sort Sun, Dongbo
collection PubMed
description Seven overlapping truncated forms of the C subunit of porcine aminopeptidase N (pAPN-C) were expressed in Escherichia coli. By western blotting and ELISA test, all recombinant proteins were recognized by the antibody against native porcine aminopeptidase N. Recombinant proteins, rpAPN-C2 (aa 623–722) and rpAPN-C3 (aa 673–772), had the highest binding activity with swine transmissible gastroenteritis virus among the truncated pAPN-C recombinant proteins. The overlapping region (aa 673–722) between rpAPN-C2 and rpAPN-C3 is indicated to play a key role in viral binding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10529-011-0795-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-70877522020-03-23 Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli Sun, Dongbo Shi, Hongyan Chen, Jianfei Guo, Donghua Liu, Quan He, Xianjing Bao, Jun Wang, Yunfeng Qiu, Huaji Feng, Li Biotechnol Lett Original Research Paper Seven overlapping truncated forms of the C subunit of porcine aminopeptidase N (pAPN-C) were expressed in Escherichia coli. By western blotting and ELISA test, all recombinant proteins were recognized by the antibody against native porcine aminopeptidase N. Recombinant proteins, rpAPN-C2 (aa 623–722) and rpAPN-C3 (aa 673–772), had the highest binding activity with swine transmissible gastroenteritis virus among the truncated pAPN-C recombinant proteins. The overlapping region (aa 673–722) between rpAPN-C2 and rpAPN-C3 is indicated to play a key role in viral binding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10529-011-0795-1) contains supplementary material, which is available to authorized users. Springer Netherlands 2011-11-15 2012 /pmc/articles/PMC7087752/ /pubmed/22083718 http://dx.doi.org/10.1007/s10529-011-0795-1 Text en © Springer Science+Business Media B.V. 2011 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Research Paper
Sun, Dongbo
Shi, Hongyan
Chen, Jianfei
Guo, Donghua
Liu, Quan
He, Xianjing
Bao, Jun
Wang, Yunfeng
Qiu, Huaji
Feng, Li
Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli
title Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli
title_full Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli
title_fullStr Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli
title_full_unstemmed Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli
title_short Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli
title_sort virus-binding activity of the truncated c subunit of porcine aminopeptidase n expressed in escherichia coli
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087752/
https://www.ncbi.nlm.nih.gov/pubmed/22083718
http://dx.doi.org/10.1007/s10529-011-0795-1
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