Cargando…

From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis

Droplet-based microfluidic technologies have proved themselves to be of significant utility in the performance of high-throughput chemical and biological experiments. By encapsulating and isolating reagents within femtoliter–nanoliter droplet, millions of (bio) chemical reactions can be processed in...

Descripción completa

Detalles Bibliográficos
Autores principales: Ding, Yun, Choo, Jaebum, deMello, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087872/
https://www.ncbi.nlm.nih.gov/pubmed/32214953
http://dx.doi.org/10.1007/s10404-017-1889-4
_version_ 1783509423096856576
author Ding, Yun
Choo, Jaebum
deMello, Andrew J.
author_facet Ding, Yun
Choo, Jaebum
deMello, Andrew J.
author_sort Ding, Yun
collection PubMed
description Droplet-based microfluidic technologies have proved themselves to be of significant utility in the performance of high-throughput chemical and biological experiments. By encapsulating and isolating reagents within femtoliter–nanoliter droplet, millions of (bio) chemical reactions can be processed in a parallel fashion and on ultra-short timescales. Recent applications of such technologies to genetic analysis have suggested significant utility in low-cost, efficient and rapid workflows for DNA amplification, rare mutation detection, antibody screening and next-generation sequencing. To this end, we describe and highlight some of the most interesting recent developments and applications of droplet-based microfluidics in the broad area of nucleic acid analysis. In addition, we also present a cursory description of some of the most essential functional components, which allow the creation of integrated and complex workflows based on flowing streams of droplets.
format Online
Article
Text
id pubmed-7087872
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-70878722020-03-23 From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis Ding, Yun Choo, Jaebum deMello, Andrew J. Microfluid Nanofluidics Research Paper Droplet-based microfluidic technologies have proved themselves to be of significant utility in the performance of high-throughput chemical and biological experiments. By encapsulating and isolating reagents within femtoliter–nanoliter droplet, millions of (bio) chemical reactions can be processed in a parallel fashion and on ultra-short timescales. Recent applications of such technologies to genetic analysis have suggested significant utility in low-cost, efficient and rapid workflows for DNA amplification, rare mutation detection, antibody screening and next-generation sequencing. To this end, we describe and highlight some of the most interesting recent developments and applications of droplet-based microfluidics in the broad area of nucleic acid analysis. In addition, we also present a cursory description of some of the most essential functional components, which allow the creation of integrated and complex workflows based on flowing streams of droplets. Springer Berlin Heidelberg 2017-03-10 2017 /pmc/articles/PMC7087872/ /pubmed/32214953 http://dx.doi.org/10.1007/s10404-017-1889-4 Text en © Springer-Verlag Berlin Heidelberg 2017 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research Paper
Ding, Yun
Choo, Jaebum
deMello, Andrew J.
From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
title From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
title_full From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
title_fullStr From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
title_full_unstemmed From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
title_short From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
title_sort from single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087872/
https://www.ncbi.nlm.nih.gov/pubmed/32214953
http://dx.doi.org/10.1007/s10404-017-1889-4
work_keys_str_mv AT dingyun fromsinglemoleculedetectiontonextgenerationsequencingmicrofluidicdropletsforhighthroughputnucleicacidanalysis
AT choojaebum fromsinglemoleculedetectiontonextgenerationsequencingmicrofluidicdropletsforhighthroughputnucleicacidanalysis
AT demelloandrewj fromsinglemoleculedetectiontonextgenerationsequencingmicrofluidicdropletsforhighthroughputnucleicacidanalysis