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Effect of monensin on the Golgi apparatus of absorptive cells in the small intestine of the rat: Morphological and cytochemical studies

The effect of short-time treatment with the ionophore monensin, administered intraluminally at concentrations of 5 and 10 μM, was studied on the Golgi apparatus of absorptive cells in the small intestine of the rat. At 2–3 min after treatment most of the Golgi stacks exhibited dilated cisternae. At...

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Autores principales: Ellinger, Adolf, Pavelka, Margit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087932/
https://www.ncbi.nlm.nih.gov/pubmed/6141849
http://dx.doi.org/10.1007/BF00213739
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author Ellinger, Adolf
Pavelka, Margit
author_facet Ellinger, Adolf
Pavelka, Margit
author_sort Ellinger, Adolf
collection PubMed
description The effect of short-time treatment with the ionophore monensin, administered intraluminally at concentrations of 5 and 10 μM, was studied on the Golgi apparatus of absorptive cells in the small intestine of the rat. At 2–3 min after treatment most of the Golgi stacks exhibited dilated cisternae. At 4–5 min stacked cisternae were absent; they were replaced by groups of smooth-surfaced vacuoles. Dilatation and vacuolization occurred in the entire stacks without preferential effect on any particular Golgi subcompartment. Monensin did not influence the cytochemical Golgi reaction of thiamine pyrophosphatase and acid phosphatase. The characteristic staining pattern of these two enzymes in all Golgi cisternae of absorptive cells in the proximal small intestine, and the reactivity restricted to trans cisternae in distal segments of the small intestine, were unchanged after treatment with monensin. In the distal small intestine, the cytochemical pattern allowed the monensin-induced vacuoles to be attributed to the former cisor trans-Golgi face. Further, the cytochemical results demonstrate that vacuolization is not restricted to the stacked cisternae, but includes the trans-most cisterna. The latter, usually located at some distance from the Golgi stacks, has been defined as belonging to the GERL system in several types of cells. The clear response to monensin, an agent that selectively affects the Golgi apparatus, indicates common properties between trans-most and stacked Golgi cisternae.
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spelling pubmed-70879322020-03-23 Effect of monensin on the Golgi apparatus of absorptive cells in the small intestine of the rat: Morphological and cytochemical studies Ellinger, Adolf Pavelka, Margit Cell Tissue Res Article The effect of short-time treatment with the ionophore monensin, administered intraluminally at concentrations of 5 and 10 μM, was studied on the Golgi apparatus of absorptive cells in the small intestine of the rat. At 2–3 min after treatment most of the Golgi stacks exhibited dilated cisternae. At 4–5 min stacked cisternae were absent; they were replaced by groups of smooth-surfaced vacuoles. Dilatation and vacuolization occurred in the entire stacks without preferential effect on any particular Golgi subcompartment. Monensin did not influence the cytochemical Golgi reaction of thiamine pyrophosphatase and acid phosphatase. The characteristic staining pattern of these two enzymes in all Golgi cisternae of absorptive cells in the proximal small intestine, and the reactivity restricted to trans cisternae in distal segments of the small intestine, were unchanged after treatment with monensin. In the distal small intestine, the cytochemical pattern allowed the monensin-induced vacuoles to be attributed to the former cisor trans-Golgi face. Further, the cytochemical results demonstrate that vacuolization is not restricted to the stacked cisternae, but includes the trans-most cisterna. The latter, usually located at some distance from the Golgi stacks, has been defined as belonging to the GERL system in several types of cells. The clear response to monensin, an agent that selectively affects the Golgi apparatus, indicates common properties between trans-most and stacked Golgi cisternae. Springer-Verlag 1984 /pmc/articles/PMC7087932/ /pubmed/6141849 http://dx.doi.org/10.1007/BF00213739 Text en © Springer-Verlag 1984 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Ellinger, Adolf
Pavelka, Margit
Effect of monensin on the Golgi apparatus of absorptive cells in the small intestine of the rat: Morphological and cytochemical studies
title Effect of monensin on the Golgi apparatus of absorptive cells in the small intestine of the rat: Morphological and cytochemical studies
title_full Effect of monensin on the Golgi apparatus of absorptive cells in the small intestine of the rat: Morphological and cytochemical studies
title_fullStr Effect of monensin on the Golgi apparatus of absorptive cells in the small intestine of the rat: Morphological and cytochemical studies
title_full_unstemmed Effect of monensin on the Golgi apparatus of absorptive cells in the small intestine of the rat: Morphological and cytochemical studies
title_short Effect of monensin on the Golgi apparatus of absorptive cells in the small intestine of the rat: Morphological and cytochemical studies
title_sort effect of monensin on the golgi apparatus of absorptive cells in the small intestine of the rat: morphological and cytochemical studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087932/
https://www.ncbi.nlm.nih.gov/pubmed/6141849
http://dx.doi.org/10.1007/BF00213739
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