A Novel Strategy for the Preparation of Codon-Optimized Truncated Ulp1 and its Simplified Application to Cleavage the SUMO Fusion Protein

Ubiquitin-like protease 1 (Ulp1) of Saccharomyces cerevisiae emerges as a fundamental tool to obtain the natural N-terminal target protein by cleavage of the small ubiquitin-related modifier (SUMO) fusion protein. However, the costly commercial Ulp1 and its complicated procedures limit its applicati...

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Detalles Bibliográficos
Autores principales: Wang, Xiaohua, Liu, Haifeng, Liu, Yawei, Li, Yuting, Yan, Lei, Yuan, Xiaohuan, Zhang, Yufei, Wu, Yan, Liu, Jieting, Zhang, Chunlei, Chu, Yanhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088175/
https://www.ncbi.nlm.nih.gov/pubmed/26960810
http://dx.doi.org/10.1007/s10930-016-9654-1

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