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A Novel Strategy for the Preparation of Codon-Optimized Truncated Ulp1 and its Simplified Application to Cleavage the SUMO Fusion Protein
Ubiquitin-like protease 1 (Ulp1) of Saccharomyces cerevisiae emerges as a fundamental tool to obtain the natural N-terminal target protein by cleavage of the small ubiquitin-related modifier (SUMO) fusion protein. However, the costly commercial Ulp1 and its complicated procedures limit its applicati...
Autores principales: | Wang, Xiaohua, Liu, Haifeng, Liu, Yawei, Li, Yuting, Yan, Lei, Yuan, Xiaohuan, Zhang, Yufei, Wu, Yan, Liu, Jieting, Zhang, Chunlei, Chu, Yanhui |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088175/ https://www.ncbi.nlm.nih.gov/pubmed/26960810 http://dx.doi.org/10.1007/s10930-016-9654-1 |
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