Cargando…
Human melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13
Aminopeptidase N/CD13 is a Zn(2+)-dependent exoprotease present on the cell surface as a transmembrane protein. Our previous studies using aminopeptidase inhibitors and antibodies demonstrated that aminopeptidase N is involved in the degradation and invasion of the extracellular matrix (ECM) by meta...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kluwer Academic Publishers
1995
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088232/ https://www.ncbi.nlm.nih.gov/pubmed/7641419 http://dx.doi.org/10.1007/BF00121910 |
_version_ | 1783509499235008512 |
---|---|
author | Fujii, Hideji Nakajima, Motowo Saiki, Ikuo Yoneda, Junya Azuma, Ichiro Tsuruo, Takashi |
author_facet | Fujii, Hideji Nakajima, Motowo Saiki, Ikuo Yoneda, Junya Azuma, Ichiro Tsuruo, Takashi |
author_sort | Fujii, Hideji |
collection | PubMed |
description | Aminopeptidase N/CD13 is a Zn(2+)-dependent exoprotease present on the cell surface as a transmembrane protein. Our previous studies using aminopeptidase inhibitors and antibodies demonstrated that aminopeptidase N is involved in the degradation and invasion of the extracellular matrix (ECM) by metastatic tumor cells. In the present study we transfected human A375M melanoma cells with eukaryotic plasmid expression vectors that contained full length cDNA of aminopeptidase N/CD13 and examined their characteristics. The transfectants that expressed extremely high levels of aminopeptidase N/CD13 degraded type IV collagen and invaded ECM more actively than the parental and control vector-transfected cells. Furthermore, the aminopeptidase N/CD13-transfected A375M cells had significantly augmented lung colonizing potential in nude mice. The results show that the aminopeptidase N/CD13 plays an active role in degradation and invasion of ECM and may be involved in the molecular mechanisms of blood-borne metastasis. |
format | Online Article Text |
id | pubmed-7088232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | Kluwer Academic Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-70882322020-03-23 Human melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13 Fujii, Hideji Nakajima, Motowo Saiki, Ikuo Yoneda, Junya Azuma, Ichiro Tsuruo, Takashi Clin Exp Metastasis Article Aminopeptidase N/CD13 is a Zn(2+)-dependent exoprotease present on the cell surface as a transmembrane protein. Our previous studies using aminopeptidase inhibitors and antibodies demonstrated that aminopeptidase N is involved in the degradation and invasion of the extracellular matrix (ECM) by metastatic tumor cells. In the present study we transfected human A375M melanoma cells with eukaryotic plasmid expression vectors that contained full length cDNA of aminopeptidase N/CD13 and examined their characteristics. The transfectants that expressed extremely high levels of aminopeptidase N/CD13 degraded type IV collagen and invaded ECM more actively than the parental and control vector-transfected cells. Furthermore, the aminopeptidase N/CD13-transfected A375M cells had significantly augmented lung colonizing potential in nude mice. The results show that the aminopeptidase N/CD13 plays an active role in degradation and invasion of ECM and may be involved in the molecular mechanisms of blood-borne metastasis. Kluwer Academic Publishers 1995 /pmc/articles/PMC7088232/ /pubmed/7641419 http://dx.doi.org/10.1007/BF00121910 Text en © Rapid Science Publishers 1995 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Fujii, Hideji Nakajima, Motowo Saiki, Ikuo Yoneda, Junya Azuma, Ichiro Tsuruo, Takashi Human melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13 |
title | Human melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13 |
title_full | Human melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13 |
title_fullStr | Human melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13 |
title_full_unstemmed | Human melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13 |
title_short | Human melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13 |
title_sort | human melanoma invasion and metastasis enhancement by high expression of aminopeptidase n/cd13 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088232/ https://www.ncbi.nlm.nih.gov/pubmed/7641419 http://dx.doi.org/10.1007/BF00121910 |
work_keys_str_mv | AT fujiihideji humanmelanomainvasionandmetastasisenhancementbyhighexpressionofaminopeptidasencd13 AT nakajimamotowo humanmelanomainvasionandmetastasisenhancementbyhighexpressionofaminopeptidasencd13 AT saikiikuo humanmelanomainvasionandmetastasisenhancementbyhighexpressionofaminopeptidasencd13 AT yonedajunya humanmelanomainvasionandmetastasisenhancementbyhighexpressionofaminopeptidasencd13 AT azumaichiro humanmelanomainvasionandmetastasisenhancementbyhighexpressionofaminopeptidasencd13 AT tsuruotakashi humanmelanomainvasionandmetastasisenhancementbyhighexpressionofaminopeptidasencd13 |