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Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum
Asarum caudigerum (Aristolochiaceae) is a paleoherb species that is important for research in origin and evolution of angiosperm flowers due to its basal position in the angiosperm phylogeny. In this study, a subtracted floral cDNA library from floral buds of A. caudigerum was constructed and cDNA a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088318/ https://www.ncbi.nlm.nih.gov/pubmed/19904556 http://dx.doi.org/10.1007/s00425-009-1048-6 |
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author | Zhao, Yin-He Möller, Michael Yang, Jun-Bo Liu, Ting-Song Zhao, Jin-Feng Dong, Li-Na Zhang, Jin-Peng Li, Cheng-Yun Wang, Guo-Ying Li, De-Zhu |
author_facet | Zhao, Yin-He Möller, Michael Yang, Jun-Bo Liu, Ting-Song Zhao, Jin-Feng Dong, Li-Na Zhang, Jin-Peng Li, Cheng-Yun Wang, Guo-Ying Li, De-Zhu |
author_sort | Zhao, Yin-He |
collection | PubMed |
description | Asarum caudigerum (Aristolochiaceae) is a paleoherb species that is important for research in origin and evolution of angiosperm flowers due to its basal position in the angiosperm phylogeny. In this study, a subtracted floral cDNA library from floral buds of A. caudigerum was constructed and cDNA arrays by suppression subtractive hybridization were generated. cDNAs of floral buds at different stages before flower opening and of leaves at the seedling stage were used. The macroarray analyses of expression profiles of isolated floral genes showed that 157 genes out of the 612 unique ESTs tested revealed higher transcript abundance in the floral buds and uppermost leaves. Among them, 78 genes were determined to be differentially expressed in the perianth, 62 in the stamens, and 100 genes in the carpels. Quantitative real-time PCR of selected genes validated the macroarray results. Remarkably, APETALA3 (AP3) B-class genes isolated from A. caudigerum were upregulated in the perianth, stamens and carpels, implying that the expression domain of B-class genes in this basal angiosperm was broader than those in their eudicot counterparts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-009-1048-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7088318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-70883182020-03-23 Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum Zhao, Yin-He Möller, Michael Yang, Jun-Bo Liu, Ting-Song Zhao, Jin-Feng Dong, Li-Na Zhang, Jin-Peng Li, Cheng-Yun Wang, Guo-Ying Li, De-Zhu Planta Original Article Asarum caudigerum (Aristolochiaceae) is a paleoherb species that is important for research in origin and evolution of angiosperm flowers due to its basal position in the angiosperm phylogeny. In this study, a subtracted floral cDNA library from floral buds of A. caudigerum was constructed and cDNA arrays by suppression subtractive hybridization were generated. cDNAs of floral buds at different stages before flower opening and of leaves at the seedling stage were used. The macroarray analyses of expression profiles of isolated floral genes showed that 157 genes out of the 612 unique ESTs tested revealed higher transcript abundance in the floral buds and uppermost leaves. Among them, 78 genes were determined to be differentially expressed in the perianth, 62 in the stamens, and 100 genes in the carpels. Quantitative real-time PCR of selected genes validated the macroarray results. Remarkably, APETALA3 (AP3) B-class genes isolated from A. caudigerum were upregulated in the perianth, stamens and carpels, implying that the expression domain of B-class genes in this basal angiosperm was broader than those in their eudicot counterparts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-009-1048-6) contains supplementary material, which is available to authorized users. Springer-Verlag 2009-11-11 2010 /pmc/articles/PMC7088318/ /pubmed/19904556 http://dx.doi.org/10.1007/s00425-009-1048-6 Text en © Springer-Verlag 2009 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Zhao, Yin-He Möller, Michael Yang, Jun-Bo Liu, Ting-Song Zhao, Jin-Feng Dong, Li-Na Zhang, Jin-Peng Li, Cheng-Yun Wang, Guo-Ying Li, De-Zhu Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum |
title | Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum |
title_full | Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum |
title_fullStr | Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum |
title_full_unstemmed | Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum |
title_short | Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum |
title_sort | extended expression of b-class mads-box genes in the paleoherb asarum caudigerum |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088318/ https://www.ncbi.nlm.nih.gov/pubmed/19904556 http://dx.doi.org/10.1007/s00425-009-1048-6 |
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