Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice

The immunogenicity of SARS-CoV nucleocapsid DNA vaccine and the immunoregulatory activity of interleukin-2 (IL-2) were investigated. DNA vaccine plasmids, pcDNA-N and pcDNA-IL2, were constructed and inoculated into BALB/c mice with or without pcDNA-IL2 by intramuscular injection. Cellular and humora...

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Autores principales: Hu, Hui, Tao, Ling, Wang, Yabin, Chen, Liying, Yang, Jihong, Wang, Hanzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2009
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088336/
https://www.ncbi.nlm.nih.gov/pubmed/19579009
http://dx.doi.org/10.1007/s10529-009-0061-y
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author Hu, Hui
Tao, Ling
Wang, Yabin
Chen, Liying
Yang, Jihong
Wang, Hanzhong
author_facet Hu, Hui
Tao, Ling
Wang, Yabin
Chen, Liying
Yang, Jihong
Wang, Hanzhong
author_sort Hu, Hui
collection PubMed
description The immunogenicity of SARS-CoV nucleocapsid DNA vaccine and the immunoregulatory activity of interleukin-2 (IL-2) were investigated. DNA vaccine plasmids, pcDNA-N and pcDNA-IL2, were constructed and inoculated into BALB/c mice with or without pcDNA-IL2 by intramuscular injection. Cellular and humoral immune responses were assessed by indirect ELISA, lymphocyte proliferation assays, ELISPOT and FACS. The nucleocapsid DNA vaccine had good immunogenicity and can induce specific humoral and cellular immunity in BALB/c mice, while IL-2 plays an immunoadjuvant role and enhances specific immune responses. This study provides a frame of reference for the design of DNA vaccines against SARS-CoV.
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spelling pubmed-70883362020-03-23 Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice Hu, Hui Tao, Ling Wang, Yabin Chen, Liying Yang, Jihong Wang, Hanzhong Biotechnol Lett Original Research Paper The immunogenicity of SARS-CoV nucleocapsid DNA vaccine and the immunoregulatory activity of interleukin-2 (IL-2) were investigated. DNA vaccine plasmids, pcDNA-N and pcDNA-IL2, were constructed and inoculated into BALB/c mice with or without pcDNA-IL2 by intramuscular injection. Cellular and humoral immune responses were assessed by indirect ELISA, lymphocyte proliferation assays, ELISPOT and FACS. The nucleocapsid DNA vaccine had good immunogenicity and can induce specific humoral and cellular immunity in BALB/c mice, while IL-2 plays an immunoadjuvant role and enhances specific immune responses. This study provides a frame of reference for the design of DNA vaccines against SARS-CoV. Springer Netherlands 2009-07-05 2009 /pmc/articles/PMC7088336/ /pubmed/19579009 http://dx.doi.org/10.1007/s10529-009-0061-y Text en © Springer Science+Business Media B.V. 2009 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Research Paper
Hu, Hui
Tao, Ling
Wang, Yabin
Chen, Liying
Yang, Jihong
Wang, Hanzhong
Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice
title Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice
title_full Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice
title_fullStr Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice
title_full_unstemmed Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice
title_short Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice
title_sort enhancing immune responses against sars-cov nucleocapsid dna vaccine by co-inoculating interleukin-2 expressing vector in mice
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088336/
https://www.ncbi.nlm.nih.gov/pubmed/19579009
http://dx.doi.org/10.1007/s10529-009-0061-y
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