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The effect of gramicidin inclusions on the local order of membrane components

ABSTRACT. We study the local effect of the antimicrobial peptide Gramicidin A on bilayers composed of lipids or surfactants using nuclear magnetic resonance spectroscopy and wide-angle X-ray scattering, techniques that probe the orientational and positional order of the alkyl chains, respectively. T...

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Detalles Bibliográficos
Autores principales: Azar, Elise, Constantin, Doru, Warschawski, Dror E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088371/
https://www.ncbi.nlm.nih.gov/pubmed/29582214
http://dx.doi.org/10.1140/epje/i2018-11644-5
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author Azar, Elise
Constantin, Doru
Warschawski, Dror E.
author_facet Azar, Elise
Constantin, Doru
Warschawski, Dror E.
author_sort Azar, Elise
collection PubMed
description ABSTRACT. We study the local effect of the antimicrobial peptide Gramicidin A on bilayers composed of lipids or surfactants using nuclear magnetic resonance spectroscopy and wide-angle X-ray scattering, techniques that probe the orientational and positional order of the alkyl chains, respectively. The two types of order vary with temperature and peptide concentration in complex ways which depend on the membrane composition, highlighting the subtlety of the interaction between inclusions and the host bilayer. The amplitude of the variation is relatively low, indicating that the macroscopic constants used to describe the elasticity of the bilayer are unlikely to change with the addition of peptide. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material in the form of a .pdf file available from the Journal web page at 10.1140/epje/i2018-11644-5 and accessible for authorised users.
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spelling pubmed-70883712020-03-23 The effect of gramicidin inclusions on the local order of membrane components Azar, Elise Constantin, Doru Warschawski, Dror E. Eur Phys J E Soft Matter Regular Article ABSTRACT. We study the local effect of the antimicrobial peptide Gramicidin A on bilayers composed of lipids or surfactants using nuclear magnetic resonance spectroscopy and wide-angle X-ray scattering, techniques that probe the orientational and positional order of the alkyl chains, respectively. The two types of order vary with temperature and peptide concentration in complex ways which depend on the membrane composition, highlighting the subtlety of the interaction between inclusions and the host bilayer. The amplitude of the variation is relatively low, indicating that the macroscopic constants used to describe the elasticity of the bilayer are unlikely to change with the addition of peptide. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material in the form of a .pdf file available from the Journal web page at 10.1140/epje/i2018-11644-5 and accessible for authorised users. Springer Berlin Heidelberg 2018-03-28 2018 /pmc/articles/PMC7088371/ /pubmed/29582214 http://dx.doi.org/10.1140/epje/i2018-11644-5 Text en © EDP Sciences, SIF, Springer-Verlag GmbH Germany, part of Springer Nature 2018 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Regular Article
Azar, Elise
Constantin, Doru
Warschawski, Dror E.
The effect of gramicidin inclusions on the local order of membrane components
title The effect of gramicidin inclusions on the local order of membrane components
title_full The effect of gramicidin inclusions on the local order of membrane components
title_fullStr The effect of gramicidin inclusions on the local order of membrane components
title_full_unstemmed The effect of gramicidin inclusions on the local order of membrane components
title_short The effect of gramicidin inclusions on the local order of membrane components
title_sort effect of gramicidin inclusions on the local order of membrane components
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088371/
https://www.ncbi.nlm.nih.gov/pubmed/29582214
http://dx.doi.org/10.1140/epje/i2018-11644-5
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