An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen

Two new enzyme-linked immunosorbent assayes (ELISA) for the diagnosis ofBabesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The seco...

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Detalles Bibliográficos
Autores principales: Böse, R., Jacobson, R. H., Gale, K. R., Waltisbuhl, D. J., Wright, I. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1990
Materias:
Original Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088413/
https://www.ncbi.nlm.nih.gov/pubmed/2251239
http://dx.doi.org/10.1007/BF00931081
Descripción
Sumario:Two new enzyme-linked immunosorbent assayes (ELISA) for the diagnosis ofBabesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The second ELISA, using a recombinantB. bovis antigen expressed inEscherichia coli, was both sensitive and specific. It is suitable to replace the native antigen, thus avoiding large batch-to-batch variations in antigen preparations and the need to sacrifice experimental cattle.

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