An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen

Two new enzyme-linked immunosorbent assayes (ELISA) for the diagnosis ofBabesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The seco...

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Detalles Bibliográficos
Autores principales: Böse, R., Jacobson, R. H., Gale, K. R., Waltisbuhl, D. J., Wright, I. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1990
Materias:
Original Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088413/
https://www.ncbi.nlm.nih.gov/pubmed/2251239
http://dx.doi.org/10.1007/BF00931081
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author Böse, R.
Jacobson, R. H.
Gale, K. R.
Waltisbuhl, D. J.
Wright, I. G.
author_facet Böse, R.
Jacobson, R. H.
Gale, K. R.
Waltisbuhl, D. J.
Wright, I. G.
author_sort Böse, R.
collection PubMed
description Two new enzyme-linked immunosorbent assayes (ELISA) for the diagnosis ofBabesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The second ELISA, using a recombinantB. bovis antigen expressed inEscherichia coli, was both sensitive and specific. It is suitable to replace the native antigen, thus avoiding large batch-to-batch variations in antigen preparations and the need to sacrifice experimental cattle.
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spelling pubmed-70884132020-03-23 An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen Böse, R. Jacobson, R. H. Gale, K. R. Waltisbuhl, D. J. Wright, I. G. Parasitol Res Original Investigations Two new enzyme-linked immunosorbent assayes (ELISA) for the diagnosis ofBabesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The second ELISA, using a recombinantB. bovis antigen expressed inEscherichia coli, was both sensitive and specific. It is suitable to replace the native antigen, thus avoiding large batch-to-batch variations in antigen preparations and the need to sacrifice experimental cattle. Springer-Verlag 1990 /pmc/articles/PMC7088413/ /pubmed/2251239 http://dx.doi.org/10.1007/BF00931081 Text en © Springer-Verlag 1990 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Investigations
Böse, R.
Jacobson, R. H.
Gale, K. R.
Waltisbuhl, D. J.
Wright, I. G.
An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen
title An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen
title_full An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen
title_fullStr An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen
title_full_unstemmed An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen
title_short An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen
title_sort improved elisa for the detection of antibodies againstbabesia bovis using either a native or a recombinantb. bovis antigen
topic Original Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088413/
https://www.ncbi.nlm.nih.gov/pubmed/2251239
http://dx.doi.org/10.1007/BF00931081
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