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Expression of SARS-coronavirus spike glycoprotein in Pichia pastoris
To establish a rapid and economical method for the expression of viral proteins in high yield and purity by Pichia pastoris, the S protein of the SARS-CoV was selected in this study. Six S glycoprotein fragments were expressed in Escherichia coli BL21 and yeast KM71H strains. After purification by a...
Autores principales: | Chuck, Chi-Pang, Wong, Chi-Hang, Chow, Larry Ming-Cheung, Fung, Kwok-Pui, Waye, Mary Miu-Yee, Tsui, Stephen Kwok-Wing |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088578/ https://www.ncbi.nlm.nih.gov/pubmed/18958613 http://dx.doi.org/10.1007/s11262-008-0292-3 |
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