The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen

The results of detection and identification of Bacillus anthracis strains in loop-mediated isothermal DNA amplification (LAMP) reaction performed under optimized conditions with original primers and thermostable DNA polymerase are presented. Reproducible LAMP-based detection of chromosomal and plasm...

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Autores principales: Shchit, I. Yu., Ignatov, K. B., Kudryavtseva, T. Yu., Shishkova, N. A., Mironova, R. I., Marinin, L. I., Mokrievich, A. N., Kramarov, V. M., Biketov, S. F., Dyatlov, I. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Allerton Press 2017
Materias:
Experimental Works
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088587/
https://www.ncbi.nlm.nih.gov/pubmed/32214650
http://dx.doi.org/10.3103/S0891416817020094
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author Shchit, I. Yu.
Ignatov, K. B.
Kudryavtseva, T. Yu.
Shishkova, N. A.
Mironova, R. I.
Marinin, L. I.
Mokrievich, A. N.
Kramarov, V. M.
Biketov, S. F.
Dyatlov, I. A.
author_facet Shchit, I. Yu.
Ignatov, K. B.
Kudryavtseva, T. Yu.
Shishkova, N. A.
Mironova, R. I.
Marinin, L. I.
Mokrievich, A. N.
Kramarov, V. M.
Biketov, S. F.
Dyatlov, I. A.
author_sort Shchit, I. Yu.
collection PubMed
description The results of detection and identification of Bacillus anthracis strains in loop-mediated isothermal DNA amplification (LAMP) reaction performed under optimized conditions with original primers and thermostable DNA polymerase are presented. Reproducible LAMP-based detection of chromosomal and plasmid DNA targets specific for B. anthracis strains has been demonstrated. No cross reactions with DNA from bacterial strains of other species of the B. cereus group were detected. The development of tests for anthrax-pathogen detection based on the optimized reaction of loop isothermal DNA amplification is planned. These tests will be convenient for clinical studies and field diagnostics due to the absence of requirements for sophisticated equipment.
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spelling pubmed-70885872020-03-23 The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen Shchit, I. Yu. Ignatov, K. B. Kudryavtseva, T. Yu. Shishkova, N. A. Mironova, R. I. Marinin, L. I. Mokrievich, A. N. Kramarov, V. M. Biketov, S. F. Dyatlov, I. A. Mol. Gen. Microbiol. Virol Experimental Works The results of detection and identification of Bacillus anthracis strains in loop-mediated isothermal DNA amplification (LAMP) reaction performed under optimized conditions with original primers and thermostable DNA polymerase are presented. Reproducible LAMP-based detection of chromosomal and plasmid DNA targets specific for B. anthracis strains has been demonstrated. No cross reactions with DNA from bacterial strains of other species of the B. cereus group were detected. The development of tests for anthrax-pathogen detection based on the optimized reaction of loop isothermal DNA amplification is planned. These tests will be convenient for clinical studies and field diagnostics due to the absence of requirements for sophisticated equipment. Allerton Press 2017-09-20 2017 /pmc/articles/PMC7088587/ /pubmed/32214650 http://dx.doi.org/10.3103/S0891416817020094 Text en © Allerton Press, Inc. 2017 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Experimental Works
Shchit, I. Yu.
Ignatov, K. B.
Kudryavtseva, T. Yu.
Shishkova, N. A.
Mironova, R. I.
Marinin, L. I.
Mokrievich, A. N.
Kramarov, V. M.
Biketov, S. F.
Dyatlov, I. A.
The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen
title The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen
title_full The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen
title_fullStr The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen
title_full_unstemmed The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen
title_short The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen
title_sort use of loop-mediated isothermal dna amplification for the detection and identification of the anthrax pathogen
topic Experimental Works
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088587/
https://www.ncbi.nlm.nih.gov/pubmed/32214650
http://dx.doi.org/10.3103/S0891416817020094
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