A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy

A simple procedure for the rapid preparation of virus isolates from cell culture for negative-contrast electron microscopy was devised. Using only conventional centrifugation steps (i.e. without ultracentrifugation), the procedure produced consistent, fine-quality preparations of a variety of virus...

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Detalles Bibliográficos
Autores principales: Skilling, D. E., Barlough, J. E., Berry, E. S., Smith, A. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers 1986
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088600/
https://www.ncbi.nlm.nih.gov/pubmed/32214599
http://dx.doi.org/10.1007/BF01665758
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author Skilling, D. E.
Barlough, J. E.
Berry, E. S.
Smith, A. W.
author_facet Skilling, D. E.
Barlough, J. E.
Berry, E. S.
Smith, A. W.
author_sort Skilling, D. E.
collection PubMed
description A simple procedure for the rapid preparation of virus isolates from cell culture for negative-contrast electron microscopy was devised. Using only conventional centrifugation steps (i.e. without ultracentrifugation), the procedure produced consistent, fine-quality preparations of a variety of virus types differing in size/shape and buoyant density.
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spelling pubmed-70886002020-03-23 A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy Skilling, D. E. Barlough, J. E. Berry, E. S. Smith, A. W. J Tissue Cult Methods Article A simple procedure for the rapid preparation of virus isolates from cell culture for negative-contrast electron microscopy was devised. Using only conventional centrifugation steps (i.e. without ultracentrifugation), the procedure produced consistent, fine-quality preparations of a variety of virus types differing in size/shape and buoyant density. Kluwer Academic Publishers 1986 /pmc/articles/PMC7088600/ /pubmed/32214599 http://dx.doi.org/10.1007/BF01665758 Text en © Tissue Culture Association, Inc. 1986 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Skilling, D. E.
Barlough, J. E.
Berry, E. S.
Smith, A. W.
A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy
title A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy
title_full A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy
title_fullStr A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy
title_full_unstemmed A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy
title_short A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy
title_sort simple, rapid method for preparation of virus isolates from cell culture for electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088600/
https://www.ncbi.nlm.nih.gov/pubmed/32214599
http://dx.doi.org/10.1007/BF01665758
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