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Using DNA Shuffling to Create Novel Infectious Bronchitis Virus S1 Genes: Implications for S1 Gene Recombination

We employed the staggered extension process (StEP) to shuffle the S1 genes from four infectious bronchitis virus (IBV) strains representing four unique serotypes. Upon creating a shuffled S1 gene library, we randomly selected 25 clones and analyzed them by DNA sequencing. In total, eleven clones con...

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Detalles Bibliográficos
Autores principales:	Callison, Scott, Hilt, Deborah, Jackwood, Mark
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Kluwer Academic Publishers 2005
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088642/ https://www.ncbi.nlm.nih.gov/pubmed/15965603 http://dx.doi.org/10.1007/s11262-004-2194-3

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