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Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine
A large quantity of SARS-CoV virus was proliferated in Vero cells, inactivated with β-propiolactone, then purified by Sepharose 4FF column chromatography to prepare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and electron microscopy. The vacci...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Science in China Press
2003
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088751/ https://www.ncbi.nlm.nih.gov/pubmed/32214708 http://dx.doi.org/10.1360/03wc464 |
| _version_ | 1783509604264574976 |
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| author | Tang, Lin Wang, Jian Qin, Ede Zhu, Qingyu Yu, Man Ding, Zhifen Shi, Huiying Cheng, Xiaojie Wang, Caiping Chang, Guohui Li, Shuangli Zhang, Xumin Chen, Xishu Yu, Jun Chen, Ze |
| author_facet | Tang, Lin Wang, Jian Qin, Ede Zhu, Qingyu Yu, Man Ding, Zhifen Shi, Huiying Cheng, Xiaojie Wang, Caiping Chang, Guohui Li, Shuangli Zhang, Xumin Chen, Xishu Yu, Jun Chen, Ze |
| author_sort | Tang, Lin |
| collection | PubMed |
| description | A large quantity of SARS-CoV virus was proliferated in Vero cells, inactivated with β-propiolactone, then purified by Sepharose 4FF column chromatography to prepare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and electron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide. |
| format | Online Article Text |
| id | pubmed-7088751 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2003 |
| publisher | Science in China Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-70887512020-03-23 Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine Tang, Lin Wang, Jian Qin, Ede Zhu, Qingyu Yu, Man Ding, Zhifen Shi, Huiying Cheng, Xiaojie Wang, Caiping Chang, Guohui Li, Shuangli Zhang, Xumin Chen, Xishu Yu, Jun Chen, Ze Chin Sci Bull Reports A large quantity of SARS-CoV virus was proliferated in Vero cells, inactivated with β-propiolactone, then purified by Sepharose 4FF column chromatography to prepare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and electron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide. Science in China Press 2003 /pmc/articles/PMC7088751/ /pubmed/32214708 http://dx.doi.org/10.1360/03wc464 Text en © Science in China Press 2003 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
| spellingShingle | Reports Tang, Lin Wang, Jian Qin, Ede Zhu, Qingyu Yu, Man Ding, Zhifen Shi, Huiying Cheng, Xiaojie Wang, Caiping Chang, Guohui Li, Shuangli Zhang, Xumin Chen, Xishu Yu, Jun Chen, Ze Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine |
| title | Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine |
| title_full | Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine |
| title_fullStr | Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine |
| title_full_unstemmed | Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine |
| title_short | Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine |
| title_sort | preparation, characterization and preliminary in vivo studies of inactivated sars-cov vaccine |
| topic | Reports |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088751/ https://www.ncbi.nlm.nih.gov/pubmed/32214708 http://dx.doi.org/10.1360/03wc464 |
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