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Development and application of a reverse transcription loop-mediated isothermal amplification method for rapid detection of Duck hepatitis A virus type 1

We developed and evaluated a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting Duck hepatitis A virus type 1 (DHAV-1). The amplification could be finished in 1 h under isothermal conditions at 63 °C by employing a set of four primers targeting the 2C gene of...

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Detalles Bibliográficos
Autores principales: Yang, Limin, Li, Jing, Bi, Yuhai, Xu, Lei, Liu, Wenjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088793/
https://www.ncbi.nlm.nih.gov/pubmed/22869367
http://dx.doi.org/10.1007/s11262-012-0798-6