Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain

The nucleocapsid protein gene of transmissible gastroenteritis virus, 1 149 bp in length, was amplified by RT-PCR from isolated strain HB06 and cloned into pMD18-T. Sequence comparison with other transmissible gastroenteritis virus (TGEV) strains selected from the Gene Bank revealed that the homolog...

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Detalles Bibliográficos
Autores principales: Fan, Jinghui, Zuo, Yuzhu, Zhao, Yuelan, Li, Tanqing, Zhang, Xiaobo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Higher Education Press 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088836/
https://www.ncbi.nlm.nih.gov/pubmed/32214985
http://dx.doi.org/10.1007/s11703-007-0060-5
Descripción
Sumario:The nucleocapsid protein gene of transmissible gastroenteritis virus, 1 149 bp in length, was amplified by RT-PCR from isolated strain HB06 and cloned into pMD18-T. Sequence comparison with other transmissible gastroenteritis virus (TGEV) strains selected from the Gene Bank revealed that the homology of N gene complete sequence shares more than 97% in nucleotide. N gene was cloned into BamHI and EcoRI multiple cloning sites of the prokaryotic expression vector pET 20 b, and named pETN. After being induced by isopropyl-β-D-thiogalactopyranoside (IPTG), the recombinant nucleocapsid protein was expressed. The result of SDS-PAGE and Western-blot showed that the recombinant nucleocapsid protein was 47 kDa and had strong positive reactions with TGEV-specific antibody.

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