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Comparison of the 5′ Leader Sequences of North American Isolates of Reference and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
The 5′ leader is documented to be an important regulatory element in many (+) ssRNA virus genome. To understand the significance of the 5′ leader RNA of PRRSV, we determined the complete leader sequences of fifteen different North American strains of PRRSV and predicted their secondary structures. V...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kluwer Academic Publishers
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088843/ https://www.ncbi.nlm.nih.gov/pubmed/11324758 http://dx.doi.org/10.1023/A:1008179726163 |
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author | Tan, Corrina Chang, Leo Shen, Shuo Liu, Ding Xiang Kwang, Jimmy |
author_facet | Tan, Corrina Chang, Leo Shen, Shuo Liu, Ding Xiang Kwang, Jimmy |
author_sort | Tan, Corrina |
collection | PubMed |
description | The 5′ leader is documented to be an important regulatory element in many (+) ssRNA virus genome. To understand the significance of the 5′ leader RNA of PRRSV, we determined the complete leader sequences of fifteen different North American strains of PRRSV and predicted their secondary structures. Viruses analysed included three reference strains and nine field strains originating from different geographic locations. To further examine the leader region, one of the field strains was adapted to grow in tissue culture, and three clones were isolated. We also predicted the secondary structures of two European strains based on their published sequences. The predicted RNA secondary structures of the leader sequences suggested the existence of three conserved domains formed by the 5′ region of the leader among the North American strains, two of which were conserved in the European strains. A variable structural domain was predicted from the 3′ region of the leader sequences of the North American strains, where all tissue culture-adapted isolates were characterized by a stem-loop while field isolates were characterized by an internal bulge within the stem-loop. |
format | Online Article Text |
id | pubmed-7088843 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Kluwer Academic Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-70888432020-03-23 Comparison of the 5′ Leader Sequences of North American Isolates of Reference and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Tan, Corrina Chang, Leo Shen, Shuo Liu, Ding Xiang Kwang, Jimmy Virus Genes Article The 5′ leader is documented to be an important regulatory element in many (+) ssRNA virus genome. To understand the significance of the 5′ leader RNA of PRRSV, we determined the complete leader sequences of fifteen different North American strains of PRRSV and predicted their secondary structures. Viruses analysed included three reference strains and nine field strains originating from different geographic locations. To further examine the leader region, one of the field strains was adapted to grow in tissue culture, and three clones were isolated. We also predicted the secondary structures of two European strains based on their published sequences. The predicted RNA secondary structures of the leader sequences suggested the existence of three conserved domains formed by the 5′ region of the leader among the North American strains, two of which were conserved in the European strains. A variable structural domain was predicted from the 3′ region of the leader sequences of the North American strains, where all tissue culture-adapted isolates were characterized by a stem-loop while field isolates were characterized by an internal bulge within the stem-loop. Kluwer Academic Publishers 2001 /pmc/articles/PMC7088843/ /pubmed/11324758 http://dx.doi.org/10.1023/A:1008179726163 Text en © Kluwer Academic Publishers 2001 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Tan, Corrina Chang, Leo Shen, Shuo Liu, Ding Xiang Kwang, Jimmy Comparison of the 5′ Leader Sequences of North American Isolates of Reference and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) |
title | Comparison of the 5′ Leader Sequences of North American Isolates of Reference and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) |
title_full | Comparison of the 5′ Leader Sequences of North American Isolates of Reference and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) |
title_fullStr | Comparison of the 5′ Leader Sequences of North American Isolates of Reference and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) |
title_full_unstemmed | Comparison of the 5′ Leader Sequences of North American Isolates of Reference and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) |
title_short | Comparison of the 5′ Leader Sequences of North American Isolates of Reference and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) |
title_sort | comparison of the 5′ leader sequences of north american isolates of reference and field strains of porcine reproductive and respiratory syndrome virus (prrsv) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088843/ https://www.ncbi.nlm.nih.gov/pubmed/11324758 http://dx.doi.org/10.1023/A:1008179726163 |
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