Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro

Purified avian infectious bronchitis virus (IBV) was used to screen a random phage display peptide library. After the fourth panning, 10 positive phages were sequenced and characterized. The phages specifically inhibited IBV infectivity in HeLa cells and blocked IBV haemagglutination. One linear pep...

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Detalles Bibliográficos
Autores principales: Peng, Bo, Chen, Hanyang, Tan, Yadi, Jin, Meilin, Chen, Huanchun, Guo, Aizhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Science China Press 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088975/
https://www.ncbi.nlm.nih.gov/pubmed/16704119
http://dx.doi.org/10.1007/s11427-006-0158-7
Descripción
Sumario:Purified avian infectious bronchitis virus (IBV) was used to screen a random phage display peptide library. After the fourth panning, 10 positive phages were sequenced and characterized. The phages specifically inhibited IBV infectivity in HeLa cells and blocked IBV haemagglutination. One linear peptide “GSH HRH VHS PFV” from the positive phages with the highest neutralization titer was synthesized and this peptide inhibited IBV infection in HeLa as well. The results may contribute to development of antiviral therapeutics for IBV and studying the determinants for viral and cell interaction.

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