Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro

Purified avian infectious bronchitis virus (IBV) was used to screen a random phage display peptide library. After the fourth panning, 10 positive phages were sequenced and characterized. The phages specifically inhibited IBV infectivity in HeLa cells and blocked IBV haemagglutination. One linear pep...

Descripción completa

Detalles Bibliográficos
Autores principales: Peng, Bo, Chen, Hanyang, Tan, Yadi, Jin, Meilin, Chen, Huanchun, Guo, Aizhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Science China Press 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088975/
https://www.ncbi.nlm.nih.gov/pubmed/16704119
http://dx.doi.org/10.1007/s11427-006-0158-7
_version_ 1783509641277210624
author Peng, Bo
Chen, Hanyang
Tan, Yadi
Jin, Meilin
Chen, Huanchun
Guo, Aizhen
author_facet Peng, Bo
Chen, Hanyang
Tan, Yadi
Jin, Meilin
Chen, Huanchun
Guo, Aizhen
author_sort Peng, Bo
collection PubMed
description Purified avian infectious bronchitis virus (IBV) was used to screen a random phage display peptide library. After the fourth panning, 10 positive phages were sequenced and characterized. The phages specifically inhibited IBV infectivity in HeLa cells and blocked IBV haemagglutination. One linear peptide “GSH HRH VHS PFV” from the positive phages with the highest neutralization titer was synthesized and this peptide inhibited IBV infection in HeLa as well. The results may contribute to development of antiviral therapeutics for IBV and studying the determinants for viral and cell interaction.
format Online
Article
Text
id pubmed-7088975
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher Science China Press
record_format MEDLINE/PubMed
spelling pubmed-70889752020-03-23 Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro Peng, Bo Chen, Hanyang Tan, Yadi Jin, Meilin Chen, Huanchun Guo, Aizhen Sci China C Life Sci Article Purified avian infectious bronchitis virus (IBV) was used to screen a random phage display peptide library. After the fourth panning, 10 positive phages were sequenced and characterized. The phages specifically inhibited IBV infectivity in HeLa cells and blocked IBV haemagglutination. One linear peptide “GSH HRH VHS PFV” from the positive phages with the highest neutralization titer was synthesized and this peptide inhibited IBV infection in HeLa as well. The results may contribute to development of antiviral therapeutics for IBV and studying the determinants for viral and cell interaction. Science China Press 2006-03-25 2006 /pmc/articles/PMC7088975/ /pubmed/16704119 http://dx.doi.org/10.1007/s11427-006-0158-7 Text en © Science in China Press 2006 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Peng, Bo
Chen, Hanyang
Tan, Yadi
Jin, Meilin
Chen, Huanchun
Guo, Aizhen
Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro
title Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro
title_full Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro
title_fullStr Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro
title_full_unstemmed Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro
title_short Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro
title_sort identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088975/
https://www.ncbi.nlm.nih.gov/pubmed/16704119
http://dx.doi.org/10.1007/s11427-006-0158-7
work_keys_str_mv AT pengbo identificationofonepeptidewhichinhibitedinfectivityofavianinfectiousbronchitisvirusinvitro
AT chenhanyang identificationofonepeptidewhichinhibitedinfectivityofavianinfectiousbronchitisvirusinvitro
AT tanyadi identificationofonepeptidewhichinhibitedinfectivityofavianinfectiousbronchitisvirusinvitro
AT jinmeilin identificationofonepeptidewhichinhibitedinfectivityofavianinfectiousbronchitisvirusinvitro
AT chenhuanchun identificationofonepeptidewhichinhibitedinfectivityofavianinfectiousbronchitisvirusinvitro
AT guoaizhen identificationofonepeptidewhichinhibitedinfectivityofavianinfectiousbronchitisvirusinvitro

Ejemplares similares