Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine

The rapid growth of the global HIV/AIDS epidemic makes it a high priority to develop an effective vaccine. Since a live attenuated or inactivated HIV vaccine is not likely to be approved for clinical application due to safety concerns, HIV virus like particles (VLPs) offer an attractive alternative...

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Autores principales: Zhang, XiZhen, Wang, XiaoDan, Zhao, DongHai, Meng, XiangYu, Zhao, XingHong, Yu, XiangHui, Kong, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Science China Press 2011
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089044/
https://www.ncbi.nlm.nih.gov/pubmed/22173311
http://dx.doi.org/10.1007/s11427-011-4244-0
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author Zhang, XiZhen
Wang, XiaoDan
Zhao, DongHai
Meng, XiangYu
Zhao, XingHong
Yu, XiangHui
Kong, Wei
author_facet Zhang, XiZhen
Wang, XiaoDan
Zhao, DongHai
Meng, XiangYu
Zhao, XingHong
Yu, XiangHui
Kong, Wei
author_sort Zhang, XiZhen
collection PubMed
description The rapid growth of the global HIV/AIDS epidemic makes it a high priority to develop an effective vaccine. Since a live attenuated or inactivated HIV vaccine is not likely to be approved for clinical application due to safety concerns, HIV virus like particles (VLPs) offer an attractive alternative because they are considered safer since they lack viral genome. We got a stable eukaryotic cell line by G418 resistance selection, engineered to express the HIV-1 structure protein Gag and Env efficiently and stably. We confirmed the presence of Gag and Env proteins in the cell culture supernatant and that they could self-assemble into VLPs. These VLPs were found to be able to elicit specific humoral and cellular immune response after immunization without any adjuvant.
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spelling pubmed-70890442020-03-23 Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine Zhang, XiZhen Wang, XiaoDan Zhao, DongHai Meng, XiangYu Zhao, XingHong Yu, XiangHui Kong, Wei Sci China Life Sci Research Papers The rapid growth of the global HIV/AIDS epidemic makes it a high priority to develop an effective vaccine. Since a live attenuated or inactivated HIV vaccine is not likely to be approved for clinical application due to safety concerns, HIV virus like particles (VLPs) offer an attractive alternative because they are considered safer since they lack viral genome. We got a stable eukaryotic cell line by G418 resistance selection, engineered to express the HIV-1 structure protein Gag and Env efficiently and stably. We confirmed the presence of Gag and Env proteins in the cell culture supernatant and that they could self-assemble into VLPs. These VLPs were found to be able to elicit specific humoral and cellular immune response after immunization without any adjuvant. Science China Press 2011-12-16 2011 /pmc/articles/PMC7089044/ /pubmed/22173311 http://dx.doi.org/10.1007/s11427-011-4244-0 Text en © The Author(s) 2011 Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited. Open AccessThis is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Research Papers
Zhang, XiZhen
Wang, XiaoDan
Zhao, DongHai
Meng, XiangYu
Zhao, XingHong
Yu, XiangHui
Kong, Wei
Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine
title Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine
title_full Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine
title_fullStr Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine
title_full_unstemmed Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine
title_short Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine
title_sort design and immunogenicity assessment of hiv-1 virus-like particles as a candidate vaccine
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089044/
https://www.ncbi.nlm.nih.gov/pubmed/22173311
http://dx.doi.org/10.1007/s11427-011-4244-0
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