Study of measles virus recombinant proteins and their immunobiological properties

Recombinant proteins rN (nucleocapsid) and rH/Nh (hemagglutinin) of the measles virus strain NovO/96 of genotype A were obtained. The immunobiological properties of the proteins were studied in the reaction with a panel of positive and negative sera. BALB/c mice were immunized with recombinant prote...

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Detalles Bibliográficos
Autores principales: Kulak, M. V., Netesova, N. A., Belavin, P. A., Seregina, E. V., Ignatyev, G. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Allerton Press, Inc. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089051/
https://www.ncbi.nlm.nih.gov/pubmed/32214646
http://dx.doi.org/10.1007/s11965-008-1007-2
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author Kulak, M. V.
Netesova, N. A.
Belavin, P. A.
Seregina, E. V.
Ignatyev, G. M.
author_facet Kulak, M. V.
Netesova, N. A.
Belavin, P. A.
Seregina, E. V.
Ignatyev, G. M.
author_sort Kulak, M. V.
collection PubMed
description Recombinant proteins rN (nucleocapsid) and rH/Nh (hemagglutinin) of the measles virus strain NovO/96 of genotype A were obtained. The immunobiological properties of the proteins were studied in the reaction with a panel of positive and negative sera. BALB/c mice were immunized with recombinant proteins and native antigen of the measles virus strain NovO/96 in order to obtain hyperimmune serum and its analysis using ELISA (enzyme-linked immunosorbent assay) and PRN (plaque reduction neutralization). The hyperimmune sera against recombinant proteins and native antigen of the measles virus strain NovO/96 were found to be highly active in ELISA. The antibodies against the proteins rN and rH/Nh were found to be capable of neutralizing the virus in titer 1 : 13.5 and 1 : 22.9, respectively. The neutralization titer of the antibodies generated against native antigen of the measles virus strain NovO/96 was 1 : 25.7.
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spelling pubmed-70890512020-03-23 Study of measles virus recombinant proteins and their immunobiological properties Kulak, M. V. Netesova, N. A. Belavin, P. A. Seregina, E. V. Ignatyev, G. M. Mol. Gen. Microbiol. Virol Article Recombinant proteins rN (nucleocapsid) and rH/Nh (hemagglutinin) of the measles virus strain NovO/96 of genotype A were obtained. The immunobiological properties of the proteins were studied in the reaction with a panel of positive and negative sera. BALB/c mice were immunized with recombinant proteins and native antigen of the measles virus strain NovO/96 in order to obtain hyperimmune serum and its analysis using ELISA (enzyme-linked immunosorbent assay) and PRN (plaque reduction neutralization). The hyperimmune sera against recombinant proteins and native antigen of the measles virus strain NovO/96 were found to be highly active in ELISA. The antibodies against the proteins rN and rH/Nh were found to be capable of neutralizing the virus in titer 1 : 13.5 and 1 : 22.9, respectively. The neutralization titer of the antibodies generated against native antigen of the measles virus strain NovO/96 was 1 : 25.7. Allerton Press, Inc. 2008-04-17 2008 /pmc/articles/PMC7089051/ /pubmed/32214646 http://dx.doi.org/10.1007/s11965-008-1007-2 Text en © Allerton Press, Inc. 2008 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Kulak, M. V.
Netesova, N. A.
Belavin, P. A.
Seregina, E. V.
Ignatyev, G. M.
Study of measles virus recombinant proteins and their immunobiological properties
title Study of measles virus recombinant proteins and their immunobiological properties
title_full Study of measles virus recombinant proteins and their immunobiological properties
title_fullStr Study of measles virus recombinant proteins and their immunobiological properties
title_full_unstemmed Study of measles virus recombinant proteins and their immunobiological properties
title_short Study of measles virus recombinant proteins and their immunobiological properties
title_sort study of measles virus recombinant proteins and their immunobiological properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089051/
https://www.ncbi.nlm.nih.gov/pubmed/32214646
http://dx.doi.org/10.1007/s11965-008-1007-2
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