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Molecular characterization of the 9.36 kb C-terminal region of canine coronavirus 1-71 strain

A total of 9.36 kb nucleotides of the C-terminal one-third genome of the canine Coronavirus (CCV) 1-71 strain, including all the structural protein genes and some non-structural protein (nsp) genes were cloned and sequenced. Nucleotide and amino acid sequence alignment as well as phylogenetic analys...

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Autores principales: Ma, Guanggang, Wang, Yuyan, Lu, Chengping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089161/
https://www.ncbi.nlm.nih.gov/pubmed/18306032
http://dx.doi.org/10.1007/s11262-008-0214-4
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author Ma, Guanggang
Wang, Yuyan
Lu, Chengping
author_facet Ma, Guanggang
Wang, Yuyan
Lu, Chengping
author_sort Ma, Guanggang
collection PubMed
description A total of 9.36 kb nucleotides of the C-terminal one-third genome of the canine Coronavirus (CCV) 1-71 strain, including all the structural protein genes and some non-structural protein (nsp) genes were cloned and sequenced. Nucleotide and amino acid sequence alignment as well as phylogenetic analysis of all the structural ORFs with reference coronavirus strains showed that CCV 1-71 was highly related to Chinese CCV isolates. This indicates that these CCV stains may have the same ancestor. Two large deletions were found in ORF3 and led to an elongated nsp3a and a truncated nsp3b. A single “A” insertion in a 6A stretch resulted in the truncation of nsp7b. The great variation in nsp3b and nsp7b indicates that these proteins are not essential for the viral replication.
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spelling pubmed-70891612020-03-23 Molecular characterization of the 9.36 kb C-terminal region of canine coronavirus 1-71 strain Ma, Guanggang Wang, Yuyan Lu, Chengping Virus Genes Article A total of 9.36 kb nucleotides of the C-terminal one-third genome of the canine Coronavirus (CCV) 1-71 strain, including all the structural protein genes and some non-structural protein (nsp) genes were cloned and sequenced. Nucleotide and amino acid sequence alignment as well as phylogenetic analysis of all the structural ORFs with reference coronavirus strains showed that CCV 1-71 was highly related to Chinese CCV isolates. This indicates that these CCV stains may have the same ancestor. Two large deletions were found in ORF3 and led to an elongated nsp3a and a truncated nsp3b. A single “A” insertion in a 6A stretch resulted in the truncation of nsp7b. The great variation in nsp3b and nsp7b indicates that these proteins are not essential for the viral replication. Springer US 2008-06-01 2008 /pmc/articles/PMC7089161/ /pubmed/18306032 http://dx.doi.org/10.1007/s11262-008-0214-4 Text en © Springer Science+Business Media, LLC 2008 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Ma, Guanggang
Wang, Yuyan
Lu, Chengping
Molecular characterization of the 9.36 kb C-terminal region of canine coronavirus 1-71 strain
title Molecular characterization of the 9.36 kb C-terminal region of canine coronavirus 1-71 strain
title_full Molecular characterization of the 9.36 kb C-terminal region of canine coronavirus 1-71 strain
title_fullStr Molecular characterization of the 9.36 kb C-terminal region of canine coronavirus 1-71 strain
title_full_unstemmed Molecular characterization of the 9.36 kb C-terminal region of canine coronavirus 1-71 strain
title_short Molecular characterization of the 9.36 kb C-terminal region of canine coronavirus 1-71 strain
title_sort molecular characterization of the 9.36 kb c-terminal region of canine coronavirus 1-71 strain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089161/
https://www.ncbi.nlm.nih.gov/pubmed/18306032
http://dx.doi.org/10.1007/s11262-008-0214-4
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