Generation of antibody- and B cell-deficient pigs by targeted disruption of the J-region gene segment of the heavy chain locus

A poly(A)-trap gene targeting strategy was used to disrupt the single functional heavy chain (HC) joining region (J(H)) of swine in primary fibroblasts. Genetically modified piglets were then generated via somatic cell nuclear transfer (SCNT) and bred to yield litters comprising J(H) wild-type litte...

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Detalles Bibliográficos
Autores principales: Mendicino, M., Ramsoondar, J., Phelps, C., Vaught, T., Ball, S., LeRoith, T., Monahan, J., Chen, S., Dandro, A., Boone, J., Jobst, P., Vance, A., Wertz, N., Bergman, Z., Sun, X-Z., Polejaeva, I., Butler, J., Dai, Y., Ayares, D., Wells, K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089184/
https://www.ncbi.nlm.nih.gov/pubmed/20872248
http://dx.doi.org/10.1007/s11248-010-9444-z

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