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Synthesis in Escherichia coli cells and characterization of the active exoribonuclease of severe acute respiratory syndrome coronavirus

The nsp14 protein, an exoribonuclease of the DEDD superfamily encoded by severe acute respiratory syndrome coronavirus (SARS-CoV), was expressed in fusion with different affinity tags. The recombinant nsp14 proteins with either GST fusion or 6-histidine tag were shown to possess ribonuclease activit...

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Detalles Bibliográficos
Autores principales:	Chen, P., Hu, T., Jiang, M., Guo, D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	SP MAIK Nauka/Interperiodica 2009
Materias:	Structural-Functional Analysis of Biopolymers and Their Complexes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089200/ https://www.ncbi.nlm.nih.gov/pubmed/32214468 http://dx.doi.org/10.1134/S0026893309030091

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